DOI: 10.1101/gad.269503. Inheritance of Polycomb-dependent chromosomal interactions in <i>Drosophila</i>

Inheritance of Polycomb-dependent chromosomal interactions in Drosophila

10.1101/gad.269503

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Maintenance of cell identity is a complex task that involves multiple layers of regulation, acting at all levels of chromatin packaging, from nucleosomes to folding of chromosomal domains in the cell nucleus. Polycomb-group (PcG) and trithorax-group (trxG) proteins maintain memory of chromatin states through binding at cis-regulatory elements named PcG response elements or cellular memory modules. Fab-7 is a well-defined cellular memory module involved in regulation of the homeotic gene Abdominal-B (Abd-B). In addition to its action in cis, we show here by three-dimensional FISH that the Fab-7 element leads to association of transgenes with each other or with the endogenous Fab-7, even when inserted in different chromosomes. These long-distance interactions enhance PcG-mediated silencing. They depend on PcG proteins, on DNA sequence homology, and on developmental progression. Once long-distance pairing is abolished by removal of the endogenous Fab-7, the derepressed chromatin state induced at the transgene locus can be transmitted through meiosis into a large fraction of the progeny, even after reintroduction of the endogenous Fab-7. Strikingly, meiotic inheritance of the derepressed state involves loss of pairing between endogenous and transgenic Fab-7. This suggests that transmission of nuclear architecture through cell division might contribute to inheritance of chromatin states in eukaryotes.

Bibliography

Bantignies, F., Grimaud, C., Lavrov, S., Gabut, M., & Cavalli, G. (2003). Inheritance of Polycomb-dependent chromosomal interactions in Drosophila. Genes & Development, 17(19), 2406â2420.

Authors 5

Frédéric Bantignies (first)
Charlotte Grimaud (additional)
Sergey Lavrov (additional)
Mathieu Gabut (additional)
Giacomo Cavalli (additional)

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Dates

Type	When
Created	21 years, 10 months ago (Oct. 1, 2003, 4:04 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:32 p.m.)
Indexed	2 months, 2 weeks ago (June 9, 2025, 9:06 a.m.)
Issued	21 years, 10 months ago (Oct. 1, 2003)
Published	21 years, 10 months ago (Oct. 1, 2003)
Published Online	21 years, 10 months ago (Oct. 1, 2003)
Published Print	21 years, 10 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Bantignies_2003, title={Inheritance of Polycomb-dependent chromosomal interactions in Drosophila}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.269503}, DOI={10.1101/gad.269503}, number={19}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Bantignies, Frédéric and Grimaud, Charlotte and Lavrov, Sergey and Gabut, Mathieu and Cavalli, Giacomo}, year={2003}, month=oct, pages={2406–2420} }

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    }
  },
  "alternative-id": [
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  ],
  "URL": "http://dx.doi.org/10.1101/gad.269503",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1002018.201003",
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  },
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
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        10,
        1
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}