DOI: 10.1101/gad.260403. Crystal structure of the<i>Drosophila</i>Mago nashi

Crystal structure of theDrosophilaMago nashi–Y14 complex

10.1101/gad.260403

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at ∼20 nucleotides upstream of exon–exon junctions. The EJC is a dynamic structure containing proteins functioning in the nuclear export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and Y14 are core components of the EJC, and they form a stable heterodimer that strongly associates with spliced mRNA. Here we report a 1.85 Å-resolution structure of theDrosophilaMago–Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA recognition motif (RRM) is involved in extensive protein–protein interactions with Mago. This unexpected finding provides important insights for understanding the molecular mechanisms of EJC assembly and RRM-mediated protein–protein interactions.

Bibliography

Shi, H., & Xu, R.-M. (2003). Crystal structure of theDrosophilaMago nashiâY14 complex. Genes & Development, 17(8), 971â976.

Authors 2

Hang Shi (first)
Rui-Ming Xu (additional)

References 40 Referenced 75

10.1128/MCB.22.15.5405-5418.2002
10.1107/S0907444998003254
10.1016/S0076-6879(97)77027-7
10.1093/emboj/19.12.3132
10.1016/S0960-9822(02)00902-8
10.1038/nrm760
10.1016/S1097-2765(01)00348-3
10.1016/S0960-9822(01)00508-5
10.1107/S0108767390010224 / Acta Crystallogr. A / Improved methods for binding protein models in electron density maps and the location of errors in these models. by Jones (1991)
10.1016/S1097-2765(00)00065-4
10.1093/emboj/20.22.6424
10.1016/S0092-8674(01)00480-9
10.1126/science.1062829
10.1093/emboj/20.8.2062
10.1107/S0021889891004399
10.1093/emboj/19.24.6860
10.1101/gad.14.9.1098 / Genes & Dev. / Pre-mRNA splicing alters mRNP composition: Evidence for stable association of proteins at exon–exon junctions. by Le Hir (2000)
10.1093/embo-reports/kve245
10.1093/emboj/20.17.4987
10.1038/35098106
10.1126/science.1062786
10.1016/S0092-8674(01)00202-1
10.1093/emboj/18.16.4560
10.1083/jcb.150.2.309
10.1016/S0076-6879(97)77028-9
10.1016/S0960-9822(06)00218-1
10.1093/emboj/20.14.3685
10.1242/dev.124.16.3197 / Development / Mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila. by Newmark (1997)
10.1002/prot.340110407
10.1016/S0076-6879(97)76066-X
10.1038/372432a0
10.1016/S0959-440X(00)00164-0
10.1038/29234
10.1016/S0092-8674(02)00627-X
10.1101/gad.1030602
10.1107/S0907444999000839
10.1146/annurev.biophys.27.1.407
10.1242/jcs.115.15.3033 / J. Cell Sci. / mRNA surveillance: The perfect persist. by Wagner (2002)
10.1006/geno.1999.6064
10.1038/35030160

Dates

Type	When
Created	22 years, 4 months ago (April 17, 2003, 2:16 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:05 p.m.)
Indexed	2 days, 23 hours ago (Sept. 4, 2025, 10:23 a.m.)
Issued	22 years, 4 months ago (April 15, 2003)
Published	22 years, 4 months ago (April 15, 2003)
Published Online	22 years, 4 months ago (April 15, 2003)
Published Print	22 years, 4 months ago (April 15, 2003)

Funders 0

None

BibTeX

@article{Shi_2003, title={Crystal structure of theDrosophilaMago nashi–Y14 complex}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.260403}, DOI={10.1101/gad.260403}, number={8}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Shi, Hang and Xu, Rui-Ming}, year={2003}, month=apr, pages={971–976} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T14:23:58Z",
    "timestamp": 1756995838023
  },
  "reference-count": 40,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "8",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        4,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at \u223c20 nucleotides upstream of exon\u2013exon junctions. The EJC is a dynamic structure containing proteins functioning in the nuclear export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and Y14 are core components of the EJC, and they form a stable heterodimer that strongly associates with spliced mRNA. Here we report a 1.85 \u00c5-resolution structure of the<jats:italic>Drosophila</jats:italic>Mago\u2013Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA recognition motif (RRM) is involved in extensive protein\u2013protein interactions with Mago. This unexpected finding provides important insights for understanding the molecular mechanisms of EJC assembly and RRM-mediated protein\u2013protein interactions.</jats:p>",
  "DOI": "10.1101/gad.260403",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        4,
        17
      ]
    ],
    "date-time": "2003-04-17T18:16:00Z",
    "timestamp": 1050603360000
  },
  "page": "971-976",
  "source": "Crossref",
  "is-referenced-by-count": 75,
  "title": "Crystal structure of the<i>Drosophila</i>Mago nashi\u2013Y14 complex",
  "prefix": "10.1101",
  "volume": "17",
  "author": [
    {
      "given": "Hang",
      "family": "Shi",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Rui-Ming",
      "family": "Xu",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        2003,
        4,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111519023696000_17.8.971.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.22.15.5405-5418.2002"
    },
    {
      "key": "2021111519023696000_17.8.971.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444998003254"
    },
    {
      "key": "2021111519023696000_17.8.971.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(97)77027-7"
    },
    {
      "key": "2021111519023696000_17.8.971.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.12.3132"
    },
    {
      "key": "2021111519023696000_17.8.971.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(02)00902-8"
    },
    {
      "key": "2021111519023696000_17.8.971.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm760"
    },
    {
      "key": "2021111519023696000_17.8.971.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00348-3"
    },
    {
      "key": "2021111519023696000_17.8.971.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(01)00508-5"
    },
    {
      "key": "2021111519023696000_17.8.971.9",
      "doi-asserted-by": "crossref",
      "first-page": "110",
      "DOI": "10.1107/S0108767390010224",
      "article-title": "Improved methods for binding protein models in electron density maps and the location of errors in these models.",
      "volume": "47",
      "author": "Jones",
      "year": "1991",
      "journal-title": "Acta Crystallogr. A"
    },
    {
      "key": "2021111519023696000_17.8.971.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)00065-4"
    },
    {
      "key": "2021111519023696000_17.8.971.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.22.6424"
    },
    {
      "key": "2021111519023696000_17.8.971.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00480-9"
    },
    {
      "key": "2021111519023696000_17.8.971.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1062829"
    },
    {
      "key": "2021111519023696000_17.8.971.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.8.2062"
    },
    {
      "key": "2021111519023696000_17.8.971.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0021889891004399"
    },
    {
      "key": "2021111519023696000_17.8.971.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.24.6860"
    },
    {
      "key": "2021111519023696000_17.8.971.17",
      "doi-asserted-by": "crossref",
      "first-page": "1098",
      "DOI": "10.1101/gad.14.9.1098",
      "article-title": "Pre-mRNA splicing alters mRNP composition: Evidence for stable association of proteins at exon\u2013exon junctions.",
      "volume": "14",
      "author": "Le Hir",
      "year": "2000",
      "journal-title": "Genes & Dev."
    },
    {
      "key": "2021111519023696000_17.8.971.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/embo-reports/kve245"
    },
    {
      "key": "2021111519023696000_17.8.971.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.17.4987"
    },
    {
      "key": "2021111519023696000_17.8.971.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35098106"
    },
    {
      "key": "2021111519023696000_17.8.971.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1062786"
    },
    {
      "key": "2021111519023696000_17.8.971.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00202-1"
    },
    {
      "key": "2021111519023696000_17.8.971.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/18.16.4560"
    },
    {
      "key": "2021111519023696000_17.8.971.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.150.2.309"
    },
    {
      "key": "2021111519023696000_17.8.971.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(97)77028-9"
    },
    {
      "key": "2021111519023696000_17.8.971.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(06)00218-1"
    },
    {
      "key": "2021111519023696000_17.8.971.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.14.3685"
    },
    {
      "key": "2021111519023696000_17.8.971.28",
      "doi-asserted-by": "crossref",
      "first-page": "3197",
      "DOI": "10.1242/dev.124.16.3197",
      "article-title": "Mago nashi mediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila.",
      "volume": "124",
      "author": "Newmark",
      "year": "1997",
      "journal-title": "Development"
    },
    {
      "key": "2021111519023696000_17.8.971.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/prot.340110407"
    },
    {
      "key": "2021111519023696000_17.8.971.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(97)76066-X"
    },
    {
      "key": "2021111519023696000_17.8.971.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/372432a0"
    },
    {
      "key": "2021111519023696000_17.8.971.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-440X(00)00164-0"
    },
    {
      "key": "2021111519023696000_17.8.971.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/29234"
    },
    {
      "key": "2021111519023696000_17.8.971.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00627-X"
    },
    {
      "key": "2021111519023696000_17.8.971.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1030602"
    },
    {
      "key": "2021111519023696000_17.8.971.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444999000839"
    },
    {
      "key": "2021111519023696000_17.8.971.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.biophys.27.1.407"
    },
    {
      "key": "2021111519023696000_17.8.971.38",
      "doi-asserted-by": "crossref",
      "first-page": "3033",
      "DOI": "10.1242/jcs.115.15.3033",
      "article-title": "mRNA surveillance: The perfect persist.",
      "volume": "115",
      "author": "Wagner",
      "year": "2002",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2021111519023696000_17.8.971.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1999.6064"
    },
    {
      "key": "2021111519023696000_17.8.971.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35030160"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.260403",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        16
      ]
    ],
    "date-time": "2021-11-16T03:05:17Z",
    "timestamp": 1637031917000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.260403"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        4,
        15
      ]
    ]
  },
  "references-count": 40,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          2003,
          4,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.260403"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.260403",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2003,
        4,
        15
      ]
    ]
  }
}