DOI: 10.1101/gad.222702. Targets of <i>At</i>WRKY6 regulation during plant senescence and pathogen defense

Targets of AtWRKY6 regulation during plant senescence and pathogen defense

10.1101/gad.222702

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

In Arabidopsis, WRKY factors comprise a large gene family of plant-specific transcriptional regulators controlling several types of plant stress responses. To understand the regulatory role of WRKY proteins during such processes, we identified targets of the senescence- and defense-associated WRKY6 factor. WRKY6 was found to suppress its own promoter activity as well as that of a closely related WRKY family member, indicating negative autoregulation. On the other hand, WRKY6 positively influenced the senescence- and pathogen defense-associated PR1 promoter activity, most likely involving NPR1 function. One novel identified target gene, SIRK, encodes a receptor-like protein kinase, whose developmental expression is strongly induced specifically during leaf senescence. The transcriptional activation of SIRK is dependent on WRKY6 function. Senescing leaves of wrky6 knockout mutants showed a drastic reduction, and green leaves of WRKY6 overexpression lines showed clearly elevated SIRK transcript levels. Furthermore, the SIRK gene promoter was specifically activated by WRKY6 in vivo, functioning very likely through direct W-box interactions.

Bibliography

Robatzek, S., & Somssich, I. E. (2002). Targets of AtWRKY6 regulation during plant senescence and pathogen defense. Genes & Development, 16(9), 1139â1149.

Authors 2

Silke Robatzek (first)
Imre E. Somssich (additional)

References 51 Referenced 550

10.1007/s001220050949
10.1016/S1369-5266(00)00145-X
10.1016/S0092-8674(00)81858-9
10.1073/pnas.95.11.6531
10.1046/j.1365-313x.1998.00343.x
10.1046/j.1365-313X.1999.00447.x
10.1093/nar/24.23.4624
10.1073/pnas.032485099
10.1105/tpc.12.8.1477
10.1016/1369-5266(88)80053-0
10.1046/j.1365-313x.2000.00923.x
10.1105/tpc.12.6.963
10.1093/emboj/18.17.4689
10.1016/S1360-1385(00)01600-9
10.1105/tpc.6.11.1607
10.1046/j.1365-313X.1999.00265.x
10.1093/genetics/156.1.341 / Genetics / Uncoupling salicyclic acid-dependent cell death and defense-related responses from disease resistance in the Arabidopsis mutant acd5. by Greenberg (2000)
10.1104/pp.124.3.1305
10.1016/0168-9525(96)10022-6 / Trends Genet. / Active repression mechanisms of eukaryotic transcription repressors. by Hanna-Rose (1996)
10.1007/PL00008673
10.1023/A:1005921914384
10.1101/gad.9.20.2459
10.1002/j.1460-2075.1987.tb02730.x / EMBO J. / GUS fusions: β-Glucuronidase a sensitive and versatile gene fusion marker in higher plants. by Jefferson (1987)
10.1093/emboj/19.22.6150
10.1101/gad.14.1.108 / Genes & Dev. / HAESA, an Arabidopsis leucine-rich repeat kinase, controls floral organ abscission. by Jinn (2000)
10.1007/BF00331014
10.1016/0378-1119(90)90351-Q
10.1046/j.1365-313x.1998.00288.x
10.1101/gad.831000
10.1016/S0092-8674(00)81962-5
10.1038/82521
10.1046/j.1365-313x.2000.00836.x
10.1016/S0092-8674(00)00190-2
10.1016/S0958-1669(97)80103-6
10.1042/bj3310001 / Biochem. J. / Transcriptional control and the role of silencers in transcriptional regulation in eukaryotes. by Ogbourne (1998)
10.1093/pcp/pcd028
10.1016/S0092-8674(00)00213-0
10.1023/A:1006199932265
10.1126/science.290.5499.2105
10.1046/j.1365-313X.2001.01131.x
10.1101/gad.861401
10.1016/1369-5266(88)80052-9
Rushton, P.J., Reinstädler, A., Lipka, V., Lippok, B., and Somssich, I.E. 2002. Pathogen-inducible synthetic plant promoters. Plant Cell (in press)..
Sambrook J. Fritsch E.F. Maniatis T. (1989) Molecular cloning: A laboratory manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY), 2nd ed..
10.1046/j.1365-313X.1999.00376.x
10.1073/pnas.181141598
10.1002/j.1460-2075.1990.tb07486.x / EMBO J. / A 125 bp promoter fragment is sufficient for strong elicitor-mediated gene activation in parsley. by van de Löcht (1990)
10.1002/j.1460-2075.1991.tb07702.x / EMBO J. / Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. by Weisshaar (1991)
10.1105/tpc.13.8.1779
10.1046/j.1365-313X.1999.00437.x
10.1105/tpc.13.7.1527

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 11:06 a.m.)
Indexed	3 days, 23 hours ago (Aug. 29, 2025, 5:42 a.m.)
Issued	23 years, 4 months ago (May 1, 2002)
Published	23 years, 4 months ago (May 1, 2002)
Published Online	23 years, 4 months ago (May 1, 2002)
Published Print	23 years, 4 months ago (May 1, 2002)

Funders 0

None

BibTeX

@article{Robatzek_2002, title={Targets of AtWRKY6 regulation during plant senescence and pathogen defense}, volume={16}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.222702}, DOI={10.1101/gad.222702}, number={9}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Robatzek, Silke and Somssich, Imre E.}, year={2002}, month=may, pages={1139–1149} }

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