DOI: 10.1101/gad.2.6.718. Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.

Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.

10.1101/gad.2.6.718

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Mature virions of herpes simplex virus type 1 contain an activating factor that primes transcription from the five virally encoded immediate early (IE) genes. This activator is specified by a 65-kD polypeptide termed VP16. The action of VP16 is mediated through cis-regulatory elements located in regions adjacent to each IE gene. Although VP16 is normally introduced into cells by infecting virions, its trans-activating function can also be observed by cotransfecting cells with a plasmid that encodes VP16 along with a reporter gene driven by IE cis-regulatory sequences. We have used such an assay to examine the function of mutant forms of VP16. Our results provide tentative identification of two domains of VP16 that are crucial to its role in the induction of IE gene expression. One domain is located within the carboxy-terminal 78 amino acids of VP16 and is characterized by its acidity. Another domain, located in a more amino-terminal region of the protein, appears to tailor the specificity of VP16 for IE genes. According to the results presented in this and the accompanying paper, we predict that VP16 achieves IE gene specificity via protein: protein, rather than protein: DNA, interaction.

Bibliography

Triezenberg, S. J., Kingsbury, R. C., & McKnight, S. L. (1988). Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Genes & Development, 2(6), 718â729.

Authors 3

S J Triezenberg (first)
R C Kingsbury (additional)
S L McKnight (additional)

References 33 Referenced 636

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{'key': '2021111319465148000_2.6.718.5', 'first-page': '1940', 'article-title': 'Promoter domains required for expression of plasmid-borne copies of the herpes simplex virus thymidine kinase gene in virus-infected mouse fibroblasts and microinjected frog oocytes.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Promoter domains required for expression of plasmid-borne copies of the herpes simplex virus thymidine kinase gene in virus-infected mouse fibroblasts and microinjected frog oocytes. (1985)
10.1016/0092-8674(87)90016-X
10.1016/0092-8674(85)90336-8
10.1128/jvi.38.1.50-58.1981 / J. Virol. / Cloning of herpes simplex virus type 1 sequences representing the whole genome. (1981)
{'key': '2021111319465148000_2.6.718.9', 'first-page': '1044', 'article-title': 'Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells.', 'volume': '2', 'year': '1982', 'journal-title': 'Mol. Cell Biol.'} / Mol. Cell Biol. / Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells. (1982)
{'key': '2021111319465148000_2.6.718.10', 'first-page': '1948', 'article-title': 'Delineation of transcriptional control signals within the Moloney murine sarcoma virus long terminal repeat.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Delineation of transcriptional control signals within the Moloney murine sarcoma virus long terminal repeat. (1985)
10.1128/jvi.50.3.733-738.1984 / J. Virol. / Effects of deletions on expression of the herpes simplex virus thymidine kinase gene from the intact viral genome: The amino terminus of the enzyme is dispensable for catalytic activity. (1984)
10.1128/jvi.14.1.8-19.1974 / J. Virol. / Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. (1974)
10.1016/0092-8674(86)90070-X
10.1073/pnas.81.13.4065
10.1073/pnas.84.1.71
10.1038/227680a0
10.1016/0092-8674(87)90663-5
10.1002/j.1460-2075.1984.tb01817.x / EMBO J. / Transcriptional regulation of a herpes simplex virus immediate early gene is mediated through an enhancer-type sequence. (1984)
10.1093/nar/12.14.5707
10.1016/0092-8674(87)90081-X
10.1128/jvi.44.3.939-949.1982 / J. Virol. / Structural features of the herpes simplex virus α gene 4, 0, and 27 promoter-regulatory sequences which confer α regulation on chimeric thymidine kinase genes. (1982)
10.1128/jvi.61.8.2428-2437.1987 / J. Virol. / The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1. (1987)
10.1016/S0076-6879(80)65059-9
{'key': '2021111319465148000_2.6.718.24', 'first-page': '5949', 'article-title': 'The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.', 'volume': '24', 'year': '1980', 'journal-title': 'Nucleic Acids Res.'} / Nucleic Acids Res. / The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. (1980)
10.1073/pnas.84.20.7061
10.1073/pnas.82.17.5870
10.1016/0092-8674(81)90346-9
10.1016/0042-6822(84)90238-1
10.1016/S0092-8674(88)80035-7
10.1128/jvi.54.3.879-881.1985 / J. Virol. / The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation. (1985)
10.1016/0092-8674(80)90384-0
10.1073/pnas.76.9.4350
10.1101/gad.2.6.730

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:48 p.m.)
Indexed	3 weeks ago (Aug. 5, 2025, 8:52 a.m.)
Issued	37 years, 2 months ago (June 1, 1988)
Published	37 years, 2 months ago (June 1, 1988)
Published Online	37 years, 2 months ago (June 1, 1988)
Published Print	37 years, 2 months ago (June 1, 1988)

Funders 0

None

BibTeX

@article{Triezenberg_1988, title={Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.}, volume={2}, ISSN={0890-9369}, url={http://dx.doi.org/10.1101/gad.2.6.718}, DOI={10.1101/gad.2.6.718}, number={6}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Triezenberg, S J and Kingsbury, R C and McKnight, S L}, year={1988}, month=jun, pages={718–729} }

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