DOI: 10.1101/gad.2.2.150. Changes in histone gene dosage alter transcription in yeast.

Changes in histone gene dosage alter transcription in yeast.

10.1101/gad.2.2.150

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations that inhibit adjacent gene expression. The HTA1-HTB1 locus causes suppression either when present on a high-copy-number plasmid or when mutant. These changes in HTA1-HTB1 after transcription of the genes adjacent to the delta insertions. On the basis of this result, we have examined the effects of increased and decreased histone gene dosage for all four yeast histone loci. From the types of histone gene dosage changes that cause suppression of insertion mutations, we conclude that altered stoichiometry of histone dimer sets can alter transcription in yeast.

Bibliography

Clark-Adams, C. D., Norris, D., Osley, M. A., Fassler, J. S., & Winston, F. (1988). Changes in histone gene dosage alter transcription in yeast. Genes & Development, 2(2), 150â159.

Authors 5

C D Clark-Adams (first)
D Norris (additional)
M A Osley (additional)
J S Fassler (additional)
F Winston (additional)

References 44 Referenced 202

{'key': '2021111319452952000_2.2.150.1', 'first-page': '208', 'article-title': 'Sequencing of supercoiled plasmid DNA.', 'volume': '4', 'year': '1986', 'journal-title': 'Biotechniques'} / Biotechniques / Sequencing of supercoiled plasmid DNA. (1986)
10.1093/nar/7.6.1513
10.1016/0378-1119(79)90004-0 / Gene / Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments. (1979)
{'key': '2021111319452952000_2.2.150.4', 'first-page': '458', 'article-title': 'A complementation analysis of the restriction and modification of DNA in E. coli.', 'volume': '41', 'year': '1969', 'journal-title': 'J. Mol. Biol.'} / J. Mol. Biol. / A complementation analysis of the restriction and modification of DNA in E. coli. (1969)
10.1016/0092-8674(76)90145-8
10.1016/0092-8674(82)90384-1
10.1073/pnas.79.5.1484
10.1073/pnas.81.7.1991
{'key': '2021111319452952000_2.2.150.9', 'first-page': '679', 'article-title': 'The SPT6 gene is essential for growth and is required for δ-mediated transcription in Saccharomyces cerevisiae', 'volume': '39', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The SPT6 gene is essential for growth and is required for δ-mediated transcription in Saccharomyces cerevisiae (1987)
10.1128/jb.147.2.390-400.1981 / J. Bacteriol. / Molecular cloning of chemotaxis genes and overproduction of gene products in the bacterial sensing system. (1981)
10.1093/genetics/105.4.857 / Genetics / A rapid chromosome-mapping method for cloned fragments of yeast DNA. (1983)
10.1016/0092-8674(82)90173-8
10.1093/genetics/118.2.203 / Genetics / Isolation and analysis of a novel class of suppressor of Ty insertion mutations in Saccharomyces cerevisiae. (1988)
10.1016/0022-2836(70)90303-7
10.1016/0092-8674(87)90237-6
10.1016/0092-8674(79)90237-X
10.1093/genetics/116.2.191 / Genetics / A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes. (1987)
10.1128/jb.153.1.163-168.1983 / J. Bacteriol. / Transformation of intact yeast cells treated with alkali cations. (1983)
{'key': '2021111319452952000_2.2.150.19', 'first-page': '1440', 'article-title': 'Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.', 'volume': '4', 'year': '1984', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. (1984)
10.1016/0092-8674(87)90561-7
Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1016/S0079-6603(08)60670-4 / Prog. Nucl. Acad. Res. Mol. Biol. / Structure of transcribing chromatin. (1980)
10.1016/0092-8674(86)90484-8
10.1093/genetics/105.2.327 / Genetics / Histone gene multiplicity and position effect variegation in Drosophila melanogaster (1983)
Mortimer, R.K. and D.C. Hawthorne. 1969. Yeast genetics. In The yeasts, (ed. A.H. Rose and J.S. Harrison), vol. 1, pp. 385–460. New York. Academic Press.
10.1016/0092-8674(82)90253-7
{'key': '2021111319452952000_2.2.150.27', 'first-page': '672', 'article-title': 'SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae. (1987)
{'key': '2021111319452952000_2.2.150.28', 'first-page': '3473', 'article-title': 'The two gene pairs encoding H2A and H2B play different roles in the Saccharomyces cerevisiae life cycle.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The two gene pairs encoding H2A and H2B play different roles in the Saccharomyces cerevisiae life cycle. (1987)
10.1073/pnas.78.10.6354
10.1002/yea.320010202
10.1146/annurev.cb.02.110186.001001
10.1016/0022-2836(77)90052-3
10.1016/0092-8674(80)90131-2
10.1016/0076-6879(83)01015-0
Sherman, F., G.R. Fink, and C.W. Lawrence. 1978. Methods in yeast genetics, rev. ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
{'key': '2021111319452952000_2.2.150.36', 'first-page': '1246', 'article-title': 'Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae.', 'volume': '4', 'year': '1984', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae. (1984)
10.1016/S0022-2836(83)80164-8
10.1016/S0022-2836(83)80163-6
10.1016/0042-6822(76)90382-2
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10.1016/0076-6879(83)01016-2
10.1016/0092-8674(84)90474-4
10.1093/genetics/107.2.179 / Genetics / Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. (1984)
10.1093/genetics/115.4.649 / Genetics / Three genes are required for trans-activation of Ty transcription in yeast. (1987)

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:46 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	37 years, 6 months ago (Feb. 1, 1988)
Published	37 years, 6 months ago (Feb. 1, 1988)
Published Online	37 years, 6 months ago (Feb. 1, 1988)
Published Print	37 years, 6 months ago (Feb. 1, 1988)

Funders 0

None

BibTeX

@article{Clark_Adams_1988, title={Changes in histone gene dosage alter transcription in yeast.}, volume={2}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.2.2.150}, DOI={10.1101/gad.2.2.150}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Clark-Adams, C D and Norris, D and Osley, M A and Fassler, J S and Winston, F}, year={1988}, month=feb, pages={150–159} }

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