DOI: 10.1101/gad.2.10.1258. 5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

10.1101/gad.2.10.1258

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Using a strategy of compensatory nucleotide changes between yeast U1 and a 5' splice site, we have analyzed the contribution of base-pairing to the efficiency and fidelity of pre-mRNA splicing in vivo. Watson-Crick base-pairing interactions with U1 can be demonstrated at intron positions 1 and 5 but not at position 4. Moreover, restoration of the ability to pair with U1 is not sufficient to restore activity in the second step of splicing to intron position 1 mutants. Finally, in contrast to recent observations in mammalian systems, we find that the precise position of 5' splice site cleavage is not determined solely by the base-pairing interaction with U1. Rather, the presence of a G residue at position 5 is required for the correct localization of the nucleolytic event. Taken together, these results indicate that the demands for 5' splice site selection and utilization are more complex than a simple maximization of Watson-Crick interactions with U1.

Bibliography

Siliciano, P. G., & Guthrie, C. (1988). 5â splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements. Genes & Development, 2(10), 1258â1267.

Authors 2

P G Siliciano (first)
C Guthrie (additional)

References 40 Referenced 203

10.1016/0092-8674(86)90620-3
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{'key': '2021111319483166000_2.10.1258.3', 'first-page': '102', 'article-title': 'Precision and orderliness in splicing.', 'volume': '4', 'year': '1987', 'journal-title': 'Trends Genet.'} / Trends Genet. / Precision and orderliness in splicing. (1987)
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Evnin, L.B. and Craik. C.S. 1988. Development of an efficient method for generating and screening active trypsin and trypsin variants. Ann. N.Y. Acad. Sci. (in press). (10.1111/j.1749-6632.1988.tb25808.x)
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10.1016/0092-8674(83)90107-1
{'key': '2021111319483166000_2.10.1258.39', 'first-page': '3018', 'article-title': 'The natural 5′ splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The natural 5′ splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA. (1987)
10.1016/0092-8674(86)90064-4

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:50 p.m.)
Indexed	6 days, 19 hours ago (Aug. 27, 2025, 11:36 a.m.)
Issued	36 years, 11 months ago (Oct. 1, 1988)
Published	36 years, 11 months ago (Oct. 1, 1988)
Published Online	36 years, 11 months ago (Oct. 1, 1988)
Published Print	36 years, 11 months ago (Oct. 1, 1988)

Funders 0

None

BibTeX

@article{Siliciano_1988, title={5’ splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.}, volume={2}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.2.10.1258}, DOI={10.1101/gad.2.10.1258}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Siliciano, P G and Guthrie, C}, year={1988}, month=oct, pages={1258–1267} }

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