DOI: 10.1101/gad.194936.112. Transcription initiation by human RNA polymerase II visualized at single-molecule resolution

Transcription initiation by human RNA polymerase II visualized at single-molecule resolution

10.1101/gad.194936.112

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Forty years of classical biochemical analysis have identified the molecular players involved in initiation of transcription by eukaryotic RNA polymerase II (Pol II) and largely assigned their functions. However, a dynamic picture of Pol II transcription initiation and an understanding of the mechanisms of its regulation have remained elusive due in part to inherent limitations of conventional ensemble biochemistry. Here we have begun to dissect promoter-specific transcription initiation directed by a reconstituted human Pol II system at single-molecule resolution using fluorescence video-microscopy. We detected several stochastic rounds of human Pol II transcription from individual DNA templates, observed attenuation of transcription by promoter mutations, observed enhancement of transcription by activator Sp1, and correlated the transcription signals with real-time interactions of holo-TFIID molecules at individual DNA templates. This integrated single-molecule methodology should be applicable to studying other complex biological processes.

Bibliography

Revyakin, A., Zhang, Z., Coleman, R. A., Li, Y., Inouye, C., Lucas, J. K., Park, S.-R., Chu, S., & Tjian, R. (2012). Transcription initiation by human RNA polymerase II visualized at single-molecule resolution. Genes & Development, 26(15), 1691â1702.

Authors 9

Andrey Revyakin (first)
Zhengjian Zhang (additional)
Robert A. Coleman (additional)
Yan Li (additional)
Carla Inouye (additional)
Julian K. Lucas (additional)
Sang-Ryul Park (additional)
Steven Chu (additional)
Robert Tjian (additional)

References 59 Referenced 79

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Dates

Type	When
Created	13 years, 1 month ago (July 19, 2012, 7:53 a.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 10 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 10:41 a.m.)
Issued	13 years, 1 month ago (July 18, 2012)
Published	13 years, 1 month ago (July 18, 2012)
Published Online	13 years, 1 month ago (July 18, 2012)
Published Print	13 years ago (Aug. 1, 2012)

Funders 0

None

BibTeX

@article{Revyakin_2012, title={Transcription initiation by human RNA polymerase II visualized at single-molecule resolution}, volume={26}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.194936.112}, DOI={10.1101/gad.194936.112}, number={15}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Revyakin, Andrey and Zhang, Zhengjian and Coleman, Robert A. and Li, Yan and Inouye, Carla and Lucas, Julian K. and Park, Sang-Ryul and Chu, Steven and Tjian, Robert}, year={2012}, month=jul, pages={1691–1702} }

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