DOI: 10.1101/gad.194101. Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development

10.1101/gad.194101

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

MBD2 and MBD3 are closely related proteins with consensus methyl-CpG binding domains. MBD2 is a transcriptional repressor that specifically binds to methylated DNA and is a component of the MeCP1 protein complex. In contrast, MBD3 fails to bind methylated DNA in murine cells, and is a component of the Mi-2/NuRD corepressor complex. We show by gene targeting that the two proteins are not functionally redundant in mice, as Mbd3(−/−) mice die during early embryogenesis, whereas Mbd2(−/−) mice are viable and fertile. Maternal behavior of Mbd2(−/−) mice is however defective and, at the molecular level, Mbd2(−/−) mice lack a component of MeCP1.Mbd2-mutant cells fail to fully silence transcription from exogenous methylated templates, but inappropriate activation of endogenous imprinted genes or retroviral sequences was not detected. Despite their differences, Mbd3 and Mbd2 interact genetically suggesting a functional relationship. Genetic and biochemical data together favor the view that MBD3 is a key component of the Mi-2/NuRD corepressor complex, whereas MBD2 may be one of several factors that can recruit this complex to DNA.

Bibliography

Hendrich, B., Guy, J., Ramsahoye, B., Wilson, V. A., & Bird, A. (2001). Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development. Genes & Development, 15(6), 710â723.

Authors 5

Brian Hendrich (first)
Jacqueline Guy (additional)
Bernard Ramsahoye (additional)
Valerie A. Wilson (additional)
Adrian Bird (additional)

References 58 Referenced 418

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:36 a.m.)
Indexed	6 hours, 59 minutes ago (Aug. 30, 2025, 12:33 p.m.)
Issued	24 years, 5 months ago (March 15, 2001)
Published	24 years, 5 months ago (March 15, 2001)
Published Online	24 years, 5 months ago (March 15, 2001)
Published Print	24 years, 5 months ago (March 15, 2001)

Funders 0

None

BibTeX

@article{Hendrich_2001, title={Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development}, volume={15}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.194101}, DOI={10.1101/gad.194101}, number={6}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Hendrich, Brian and Guy, Jacqueline and Ramsahoye, Bernard and Wilson, Valerie A. and Bird, Adrian}, year={2001}, month=mar, pages={710–723} }

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    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          2001,
          3,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.194101"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.194101",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2001,
        3,
        15
      ]
    ]
  }
}