DOI: 10.1101/gad.17900. <i>Drosophila</i> homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification

Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification

10.1101/gad.17900

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the Drosophila homologs of 23 human mediator genes and mutations of two, dTRAP240 and of dTRAP80 (the putative fly homolog of yeast SRB4). Clonal analysis indicates a general role for dTRAP80 necessary for cell viability. ThedTRAP240 gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic Sex combs reduced product, required for the same cell/tissue identities, as a functional partner of these mediator proteins.

Bibliography

Boube, M., Faucher, C., Joulia, L., Cribbs, D. L., & Bourbon, H.-M. (2000). Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification. Genes & Development, 14(22), 2906â2917.

Authors 5

Muriel Boube (first)
Christian Faucher (additional)
Laurent Joulia (additional)
David L. Cribbs (additional)
Henri-Marc Bourbon (additional)

References 37 Referenced 59

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{'key': '2021111419243695000_14.22.2906.16', 'first-page': '4727', 'article-title': 'Structure and expression of ubiquitin genes of Drosophila melanogaster.', 'volume': '8', 'author': 'Lee', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Structure and expression of ubiquitin genes of Drosophila melanogaster. by Lee (1988)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:37 p.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 8:28 p.m.)
Issued	24 years, 9 months ago (Nov. 15, 2000)
Published	24 years, 9 months ago (Nov. 15, 2000)
Published Online	24 years, 9 months ago (Nov. 15, 2000)
Published Print	24 years, 9 months ago (Nov. 15, 2000)

Funders 0

None

BibTeX

@article{Boube_2000, title={Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.17900}, DOI={10.1101/gad.17900}, number={22}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Boube, Muriel and Faucher, Christian and Joulia, Laurent and Cribbs, David L. and Bourbon, Henri-Marc}, year={2000}, month=nov, pages={2906–2917} }

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  "abstract": "<jats:p>The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the <jats:italic>Drosophila</jats:italic> homologs of 23 human mediator genes and mutations of two, <jats:italic>dTRAP240</jats:italic> and of <jats:italic>dTRAP80</jats:italic> (the putative fly homolog of yeast <jats:italic>SRB4</jats:italic>). Clonal analysis indicates a general role for <jats:italic>dTRAP80</jats:italic> necessary for cell viability. The<jats:italic>dTRAP240</jats:italic> gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic <jats:italic>Sex combs reduced</jats:italic> product, required for the same cell/tissue identities, as a functional partner of these mediator proteins.</jats:p>",
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