DOI: 10.1101/gad.1675708. Identification of <i>cis</i>-acting sites for condensin loading onto budding yeast chromosomes

Identification of cis-acting sites for condensin loading onto budding yeast chromosomes

10.1101/gad.1675708

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonucleotide tiling arrays. Condensin-binding sites coincide with those of the loading factor Scc2/4 of the related cohesin complex. The sites map to tRNA and other genes bound by the RNA polymerase III transcription factor TFIIIC, and ribosomal protein and SNR genes. An ectopic B-box element, recognized by TFIIIC, constitutes a minimal condensin-binding site, and TFIIIC and the Scc2/4 complex promote functional condensin association with chromosomes. A similar pattern of condensin binding is conserved along fission yeast chromosomes. This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis.

Bibliography

DâAmbrosio, C., Schmidt, C. K., Katou, Y., Kelly, G., Itoh, T., Shirahige, K., & Uhlmann, F. (2008). Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes & Development, 22(16), 2215â2227.

Authors 7

Claudio D’Ambrosio (first)
Christine Katrin Schmidt (additional)
Yuki Katou (additional)
Gavin Kelly (additional)
Takehiko Itoh (additional)
Katsuhiko Shirahige (additional)
Frank Uhlmann (additional)

References 51 Referenced 298

10.1083/jcb.200111002
10.1038/417197a
10.1016/j.cub.2003.10.036
10.1016/S1097-2765(02)00546-4
10.1016/j.molcel.2006.05.014
10.1091/mbc.01-05-0264
10.1016/S0092-8674(00)81804-8
10.1016/S1097-2765(00)80398-6
10.1101/gad.1583807
10.1101/gad.12.21.3408 / Genes & Dev. / Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G1 phase by Furuya, (1998)
10.1016/j.cub.2005.12.040
10.1016/j.cub.2006.06.068
10.1016/j.cub.2004.07.053
10.1083/jcb.125.3.517
10.1016/S1097-2765(02)00515-4
10.1101/gad.1675908
10.1038/nrm1909
10.1016/0092-8674(94)90254-2
10.1242/jcs.01604
10.1093/nar/29.13.2675
10.1016/S1534-5807(03)00199-0
10.1016/j.cell.2005.07.018
10.1111/j.1365-2443.2007.01085.x
10.1038/nature01900
10.1126/science.282.5388.487
10.1101/gad.1150404
10.1016/S0021-9258(17)31663-0 / J. Biol. Chem. / A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis by Lefebvre, (1994)
10.1038/nature02742
10.1016/S1097-2765(01)00161-7
10.1091/mbc.01-09-0441
10.1038/sj.emboj.7601793
10.1128/MCB.24.10.4118-4127.2004
10.1146/annurev.biochem.74.082803.133219
10.1016/j.cell.2006.04.028
10.1038/sj.emboj.7601562
10.1016/S0092-8674(03)00724-4
10.1091/mbc.E04-03-0242
10.1002/yea.817
10.1128/MCB.24.8.3100-3111.2004
10.1002/j.1460-2075.1994.tb06821.x / EMBO J. / Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis by Saka, (1994)
10.1093/genetics/142.4.1105 / Genetics / The rad9 gene of Coprinus cinereus encodes a proline-rich protein required for meiotic chromosome condensation and synapsis by Seitz, (1996)
10.1101/gad.9.5.587
10.1016/S0092-8674(04)00415-5
10.1101/gad.13.17.2271
10.1126/science.1089814
10.1146/annurev.genet.39.073003.113546
10.1128/MCB.25.16.7216-7225.2005
10.4161/cc.5.19.3292 / Cell Cycle / Condensin function in mitotic nucleolar segregation is regulated by rDNA transcription by Wang, (2006)
10.1016/j.cub.2006.03.049
10.1016/j.cub.2003.10.030
10.1128/EC.2.2.256-264.2003

Dates

Type	When
Created	17 years ago (Aug. 15, 2008, 1:34 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 12:17 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 12, 2025, 5:25 p.m.)
Issued	17 years ago (Aug. 15, 2008)
Published	17 years ago (Aug. 15, 2008)
Published Online	17 years ago (Aug. 15, 2008)
Published Print	17 years ago (Aug. 15, 2008)

Funders 0

None

BibTeX

@article{D_Ambrosio_2008, title={Identification of cis-acting sites for condensin loading onto budding yeast chromosomes}, volume={22}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1675708}, DOI={10.1101/gad.1675708}, number={16}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={D’Ambrosio, Claudio and Schmidt, Christine Katrin and Katou, Yuki and Kelly, Gavin and Itoh, Takehiko and Shirahige, Katsuhiko and Uhlmann, Frank}, year={2008}, month=aug, pages={2215–2227} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        12
      ]
    ],
    "date-time": "2025-08-12T21:25:09Z",
    "timestamp": 1755033909769
  },
  "reference-count": 51,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "16",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2008,
        8,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonucleotide tiling arrays. Condensin-binding sites coincide with those of the loading factor Scc2/4 of the related cohesin complex. The sites map to tRNA and other genes bound by the RNA polymerase III transcription factor TFIIIC, and ribosomal protein and <jats:italic>SNR</jats:italic> genes. An ectopic B-box element, recognized by TFIIIC, constitutes a minimal condensin-binding site, and TFIIIC and the Scc2/4 complex promote functional condensin association with chromosomes. A similar pattern of condensin binding is conserved along fission yeast chromosomes. This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis.</jats:p>",
  "DOI": "10.1101/gad.1675708",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        8,
        15
      ]
    ],
    "date-time": "2008-08-15T17:34:56Z",
    "timestamp": 1218821696000
  },
  "page": "2215-2227",
  "source": "Crossref",
  "is-referenced-by-count": 298,
  "title": "Identification of <i>cis</i>-acting sites for condensin loading onto budding yeast chromosomes",
  "prefix": "10.1101",
  "volume": "22",
  "author": [
    {
      "given": "Claudio",
      "family": "D\u2019Ambrosio",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Christine Katrin",
      "family": "Schmidt",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Yuki",
      "family": "Katou",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Gavin",
      "family": "Kelly",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Takehiko",
      "family": "Itoh",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Katsuhiko",
      "family": "Shirahige",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Frank",
      "family": "Uhlmann",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        2008,
        8,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111608072052000_22.16.2215.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200111002"
    },
    {
      "key": "2021111608072052000_22.16.2215.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/417197a"
    },
    {
      "key": "2021111608072052000_22.16.2215.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2003.10.036"
    },
    {
      "key": "2021111608072052000_22.16.2215.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(02)00546-4"
    },
    {
      "key": "2021111608072052000_22.16.2215.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.molcel.2006.05.014"
    },
    {
      "key": "2021111608072052000_22.16.2215.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.01-05-0264"
    },
    {
      "key": "2021111608072052000_22.16.2215.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81804-8"
    },
    {
      "key": "2021111608072052000_22.16.2215.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80398-6"
    },
    {
      "key": "2021111608072052000_22.16.2215.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1583807"
    },
    {
      "key": "2021111608072052000_22.16.2215.10",
      "doi-asserted-by": "crossref",
      "first-page": "3408",
      "DOI": "10.1101/gad.12.21.3408",
      "article-title": "Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G1 phase",
      "volume": "12",
      "author": "Furuya,",
      "year": "1998",
      "journal-title": "Genes & Dev."
    },
    {
      "key": "2021111608072052000_22.16.2215.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2005.12.040"
    },
    {
      "key": "2021111608072052000_22.16.2215.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2006.06.068"
    },
    {
      "key": "2021111608072052000_22.16.2215.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2004.07.053"
    },
    {
      "key": "2021111608072052000_22.16.2215.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.125.3.517"
    },
    {
      "key": "2021111608072052000_22.16.2215.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(02)00515-4"
    },
    {
      "key": "2021111608072052000_22.16.2215.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1675908"
    },
    {
      "key": "2021111608072052000_22.16.2215.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm1909"
    },
    {
      "key": "2021111608072052000_22.16.2215.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90254-2"
    },
    {
      "key": "2021111608072052000_22.16.2215.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.01604"
    },
    {
      "key": "2021111608072052000_22.16.2215.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/29.13.2675"
    },
    {
      "key": "2021111608072052000_22.16.2215.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1534-5807(03)00199-0"
    },
    {
      "key": "2021111608072052000_22.16.2215.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2005.07.018"
    },
    {
      "key": "2021111608072052000_22.16.2215.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2443.2007.01085.x"
    },
    {
      "key": "2021111608072052000_22.16.2215.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature01900"
    },
    {
      "key": "2021111608072052000_22.16.2215.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.282.5388.487"
    },
    {
      "key": "2021111608072052000_22.16.2215.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1150404"
    },
    {
      "key": "2021111608072052000_22.16.2215.27",
      "doi-asserted-by": "crossref",
      "first-page": "23374",
      "DOI": "10.1016/S0021-9258(17)31663-0",
      "article-title": "A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis",
      "volume": "269",
      "author": "Lefebvre,",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111608072052000_22.16.2215.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature02742"
    },
    {
      "key": "2021111608072052000_22.16.2215.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00161-7"
    },
    {
      "key": "2021111608072052000_22.16.2215.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.01-09-0441"
    },
    {
      "key": "2021111608072052000_22.16.2215.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.emboj.7601793"
    },
    {
      "key": "2021111608072052000_22.16.2215.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.24.10.4118-4127.2004"
    },
    {
      "key": "2021111608072052000_22.16.2215.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.biochem.74.082803.133219"
    },
    {
      "key": "2021111608072052000_22.16.2215.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2006.04.028"
    },
    {
      "key": "2021111608072052000_22.16.2215.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.emboj.7601562"
    },
    {
      "key": "2021111608072052000_22.16.2215.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(03)00724-4"
    },
    {
      "key": "2021111608072052000_22.16.2215.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.E04-03-0242"
    },
    {
      "key": "2021111608072052000_22.16.2215.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.817"
    },
    {
      "key": "2021111608072052000_22.16.2215.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.24.8.3100-3111.2004"
    },
    {
      "key": "2021111608072052000_22.16.2215.40",
      "doi-asserted-by": "crossref",
      "first-page": "4938",
      "DOI": "10.1002/j.1460-2075.1994.tb06821.x",
      "article-title": "Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis",
      "volume": "13",
      "author": "Saka,",
      "year": "1994",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111608072052000_22.16.2215.41",
      "doi-asserted-by": "crossref",
      "first-page": "1105",
      "DOI": "10.1093/genetics/142.4.1105",
      "article-title": "The rad9 gene of Coprinus cinereus encodes a proline-rich protein required for meiotic chromosome condensation and synapsis",
      "volume": "142",
      "author": "Seitz,",
      "year": "1996",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111608072052000_22.16.2215.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.5.587"
    },
    {
      "key": "2021111608072052000_22.16.2215.43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(04)00415-5"
    },
    {
      "key": "2021111608072052000_22.16.2215.44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.13.17.2271"
    },
    {
      "key": "2021111608072052000_22.16.2215.45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1089814"
    },
    {
      "key": "2021111608072052000_22.16.2215.46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.genet.39.073003.113546"
    },
    {
      "key": "2021111608072052000_22.16.2215.47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.25.16.7216-7225.2005"
    },
    {
      "key": "2021111608072052000_22.16.2215.48",
      "doi-asserted-by": "crossref",
      "first-page": "2260",
      "DOI": "10.4161/cc.5.19.3292",
      "article-title": "Condensin function in mitotic nucleolar segregation is regulated by rDNA transcription",
      "volume": "5",
      "author": "Wang,",
      "year": "2006",
      "journal-title": "Cell Cycle"
    },
    {
      "key": "2021111608072052000_22.16.2215.49",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2006.03.049"
    },
    {
      "key": "2021111608072052000_22.16.2215.50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2003.10.030"
    },
    {
      "key": "2021111608072052000_22.16.2215.51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/EC.2.2.256-264.2003"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.1675708",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        16
      ]
    ],
    "date-time": "2021-11-16T17:17:22Z",
    "timestamp": 1637083042000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.1675708"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2008,
        8,
        15
      ]
    ]
  },
  "references-count": 51,
  "journal-issue": {
    "issue": "16",
    "published-print": {
      "date-parts": [
        [
          2008,
          8,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.1675708"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.1675708",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1120582.576793",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2008,
        8,
        15
      ]
    ]
  }
}