DOI: 10.1101/gad.1557907. Introns play an essential role in splicing-dependent formation of the exon junction complex

Introns play an essential role in splicing-dependent formation of the exon junction complex

10.1101/gad.1557907

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160. This initial association of EJC components occurs in the absence of the final EJC-binding site on the exon. RNA interference (RNAi) knockdown of IBP160 arrested EJC association with cytoplasmic RNAs following nonsense-mediated decay. We propose that the intron has a crucial role in the early steps of EJC formation and is indispensable for the subsequent formation of a functional EJC.

Bibliography

Ideue, T., Sasaki, Y. T. F., Hagiwara, M., & Hirose, T. (2007). Introns play an essential role in splicing-dependent formation of the exon junction complex. Genes & Development, 21(16), 1993â1998.

Authors 4

Takashi Ideue (first)
Yasnory T.F. Sasaki (additional)
Masatoshi Hagiwara (additional)
Tetsuro Hirose (additional)

References 31 Referenced 59

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Dates

Type	When
Created	18 years ago (Aug. 3, 2007, 9:28 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 12:08 p.m.)
Indexed	1 day, 8 hours ago (Aug. 27, 2025, 11:53 a.m.)
Issued	18 years ago (Aug. 3, 2007)
Published	18 years ago (Aug. 3, 2007)
Published Online	18 years ago (Aug. 3, 2007)
Published Print	18 years ago (Aug. 15, 2007)

Funders 0

None

BibTeX

@article{Ideue_2007, title={Introns play an essential role in splicing-dependent formation of the exon junction complex}, volume={21}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1557907}, DOI={10.1101/gad.1557907}, number={16}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Ideue, Takashi and Sasaki, Yasnory T.F. and Hagiwara, Masatoshi and Hirose, Tetsuro}, year={2007}, month=aug, pages={1993–1998} }

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