DOI: 10.1101/gad.1490207. Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast

Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast

10.1101/gad.1490207

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

mRNA surveillance system represses the expression of nonstop mRNA by rapid mRNA degradation and translation repression. Here we show that the level of protein product of nonstop mRNA containing a poly(A) tail was reduced 100-fold, and this reduction was due to rapid mRNA degradation, translation repression, and protein destabilization, at least in part, by the proteasome. Insertion of a poly(A) tract upstream of a termination codon resulted in translation repression and protein destabilization, but not rapid mRNA decay. We propose that translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization.

Bibliography

Ito-Harashima, S., Kuroha, K., Tatematsu, T., & Inada, T. (2007). Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast. Genes & Development, 21(5), 519â524.

Authors 4

Sayoko Ito-Harashima (first)
Kazushige Kuroha (additional)
Tsuyako Tatematsu (additional)
Toshifumi Inada (additional)

References 25 Referenced 181

10.1146/annurev.biochem.73.011303.074032
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Dates

Type	When
Created	18 years, 5 months ago (March 7, 2007, 12:21 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 12:03 p.m.)
Indexed	4 days, 9 hours ago (Aug. 21, 2025, 2:09 p.m.)
Issued	18 years, 5 months ago (March 1, 2007)
Published	18 years, 5 months ago (March 1, 2007)
Published Online	18 years, 5 months ago (March 7, 2007)
Published Print	18 years, 5 months ago (March 1, 2007)

Funders 0

None

BibTeX

@article{Ito_Harashima_2007, title={Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast}, volume={21}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1490207}, DOI={10.1101/gad.1490207}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Ito-Harashima, Sayoko and Kuroha, Kazushige and Tatematsu, Tsuyako and Inada, Toshifumi}, year={2007}, month=mar, pages={519–524} }

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