DOI: 10.1101/gad.1457006. Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis

Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis

10.1101/gad.1457006

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Sporulation in Saccharomyces cerevisiae is a highly regulated process wherein a diploid cell gives rise to four haploid gametes. In this study we show that histone H4 Ser1 is phosphorylated (H4 S1ph) during sporulation, starting from mid-sporulation and persisting to germination, and is temporally distinct from earlier meiosis-linked H3 S10ph involved in chromosome condensation. A histone H4 S1A substitution mutant forms aberrant spores and has reduced sporulation efficiency. Deletion of sporulation-specific yeast Sps1, a member of the Ste20 family of kinases, nearly abolishes the sporulation-associated H4 S1ph modification. H4 S1ph may promote chromatin compaction, since deletion of SPS1 increases accessibility to antibody immunoprecipitation; furthermore, either deletion of Sps1 or an H4 S1A substitution results in increased DNA volume in nuclei within spores. We find H4 S1ph present during Drosophila melanogaster and mouse spermatogenesis, and similar to yeast, this modification extends late into sperm differentiation relative to H3 S10ph. Thus, H4 S1ph may be an evolutionarily ancient histone modification to mark the genome for gamete-associated packaging.

Bibliography

Krishnamoorthy, T., Chen, X., Govin, J., Cheung, W. L., Dorsey, J., Schindler, K., Winter, E., Allis, C. D., Guacci, V., Khochbin, S., Fuller, M. T., & Berger, S. L. (2006). Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis. Genes & Development, 20(18), 2580â2592.

Authors 12

Thanuja Krishnamoorthy (first)
Xin Chen (additional)
Jerome Govin (additional)
Wang L. Cheung (additional)
Jean Dorsey (additional)
Karen Schindler (additional)
Edward Winter (additional)
C. David Allis (additional)
Vincent Guacci (additional)
Saadi Khochbin (additional)
Margaret T. Fuller (additional)
Shelley L. Berger (additional)

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Dates

Type	When
Created	18 years, 11 months ago (Sept. 15, 2006, 7:10 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 11:58 a.m.)
Indexed	1 year ago (Aug. 5, 2024, 7:14 p.m.)
Issued	18 years, 11 months ago (Sept. 15, 2006)
Published	18 years, 11 months ago (Sept. 15, 2006)
Published Online	18 years, 11 months ago (Sept. 15, 2006)
Published Print	18 years, 11 months ago (Sept. 15, 2006)

Funders 0

None

BibTeX

@article{Krishnamoorthy_2006, title={Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis}, volume={20}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1457006}, DOI={10.1101/gad.1457006}, number={18}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Krishnamoorthy, Thanuja and Chen, Xin and Govin, Jerome and Cheung, Wang L. and Dorsey, Jean and Schindler, Karen and Winter, Edward and Allis, C. David and Guacci, Vincent and Khochbin, Saadi and Fuller, Margaret T. and Berger, Shelley L.}, year={2006}, month=sep, pages={2580–2592} }

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