DOI: 10.1101/gad.1412706. SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis

SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis

10.1101/gad.1412706

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The mammalian cytoplasmic protein SirT2 is a member of the Sir2 family of NAD+-dependent protein deacetylases involved in caloric restriction-dependent life span extension. We found that SirT2 and its yeast counterpart Hst2 have a strong preference for histone H4K16Ac in their deacetylation activity in vitro and in vivo. We have pinpointed the decrease in global levels of H4K16Ac during the mammalian cell cycle to the G2/M transition that coincides with SirT2 localization on chromatin. Mouse embryonic fibroblasts (MEFs) deficient for SirT2 show higher levels of H4K16Ac in mitosis, in contrast to the normal levels exhibited by SirT1-deficient MEFs. The enzymatic conversion of H4K16Ac to its deacetylated form may be pivotal to the formation of condensed chromatin. Thus, SirT2 is a major contributor to this enzymatic conversion at the time in the cell’s life cycle when condensed chromatin must be generated anew.

Bibliography

Vaquero, A., Scher, M. B., Lee, D. H., Sutton, A., Cheng, H.-L., Alt, F. W., Serrano, L., Sternglanz, R., & Reinberg, D. (2006). SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis. Genes & Development, 20(10), 1256â1261.

Authors 9

Alejandro Vaquero (first)
Michael B. Scher (additional)
Dong Hoon Lee (additional)
Ann Sutton (additional)
Hwei-Ling Cheng (additional)
Frederick W. Alt (additional)
Lourdes Serrano (additional)
Rolf Sternglanz (additional)
Danny Reinberg (additional)

References 26 Referenced 549

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Harlow E. Lane D. (1988) Antibodies: A laboratory manual. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY).
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{'key': '2021111607393065000_20.10.1256.17', 'first-page': '4111', 'article-title': 'The highly conserved N-terminal domains of histones H3 and H4 are required for normal cell cycle progression.', 'volume': '11', 'author': 'Morgan', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The highly conserved N-terminal domains of histones H3 and H4 are required for normal cell cycle progression. by Morgan (1991)
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10.1093/emboj/18.22.6448

Dates

Type	When
Created	19 years, 3 months ago (April 28, 2006, 8:50 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 11:56 a.m.)
Indexed	1 day, 10 hours ago (Aug. 22, 2025, 12:47 a.m.)
Issued	19 years, 3 months ago (April 28, 2006)
Published	19 years, 3 months ago (April 28, 2006)
Published Online	19 years, 3 months ago (April 28, 2006)
Published Print	19 years, 3 months ago (May 15, 2006)

Funders 0

None

BibTeX

@article{Vaquero_2006, title={SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis}, volume={20}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1412706}, DOI={10.1101/gad.1412706}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Vaquero, Alejandro and Scher, Michael B. and Lee, Dong Hoon and Sutton, Ann and Cheng, Hwei-Ling and Alt, Frederick W. and Serrano, Lourdes and Sternglanz, Rolf and Reinberg, Danny}, year={2006}, month=apr, pages={1256–1261} }

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