DOI: 10.1101/gad.14.6.740. A novel pairing process promoted by <i>Escherichia coli</i> RecA protein: inverse DNA and RNA strand exchange

A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange

10.1101/gad.14.6.740

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Traditionally, recombination reactions promoted by RecA-like proteins initiate by forming a nucleoprotein filament on a single-stranded DNA (ssDNA), which then pairs with homologous double-stranded DNA (dsDNA). In this paper, we describe a novel pairing process that occurs in an unconventional manner: RecA protein polymerizes along dsDNA to form an active nucleoprotein filament that can pair and exchange strands with homologous ssDNA. Our results demonstrate that this “inverse” reaction is a unique, highly efficient DNA strand exchange reaction that is not due to redistribution of RecA protein from dsDNA to the homologous ssDNA partner. Finally, we demonstrate that the RecA protein–dsDNA filament can also pair and promote strand exchange with ssRNA. This inverse RNA strand exchange reaction is likely responsible for R-loop formation that is required for recombination-dependent DNA replication.

Bibliography

Zaitsev, E. N., & Kowalczykowski, S. C. (2000). A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange. Genes & Development, 14(6), 740â749.

Authors 2

Eugene N. Zaitsev (first)
Stephen C. Kowalczykowski (additional)

References 48 Referenced 68

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Dates

Type	When
Created	3 years, 9 months ago (Nov. 14, 2021, 10:30 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:31 p.m.)
Indexed	2 months, 2 weeks ago (June 18, 2025, 11:49 a.m.)
Issued	25 years, 5 months ago (March 15, 2000)
Published	25 years, 5 months ago (March 15, 2000)
Published Online	25 years, 5 months ago (March 15, 2000)
Published Print	25 years, 5 months ago (March 15, 2000)

Funders 0

None

BibTeX

@article{Zaitsev_2000, title={A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.14.6.740}, DOI={10.1101/gad.14.6.740}, number={6}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Zaitsev, Eugene N. and Kowalczykowski, Stephen C.}, year={2000}, month=mar, pages={740–749} }

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  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2000,
        3,
        15
      ]
    ]
  }
}