DOI: 10.1101/gad.14.3.377. Repression by Suppressor of Hairless and activation by Notch are required to define a single row of <i>single-minded</i> expressing cells in the <i>Drosophila</i> embryo

Repression by Suppressor of Hairless and activation by Notch are required to define a single row of single-minded expressing cells in the Drosophila embryo

10.1101/gad.14.3.377

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Notch signal transduction appears to involve the ligand-induced intracellular processing of Notch, and the formation of a processed Notch-Suppressor of Hairless complex that binds DNA and activates the transcription of Notch target genes. This suggests that loss of eitherNotch or Su(H) activities should lead to similar cell fate changes. However, previous data indicate that, in theDrosophila blastoderm embryo, mesectoderm specification requires Notch but not Su(H) activity. The determination of the mesectodermal fate is specified by Single-minded (Sim), a transcription factor expressed in a single row of cells abutting the mesoderm. The molecular mechanisms by which the dorsoventral gradient of nuclear Dorsal establishes the single-cell wide territory of sim expression are not fully understood. We have found that Notch activity is required for simexpression in cellularizing embryos. In contrast, at this stage,Su(H) has a dual function. Su(H) activity was required to up-regulate sim expression in the mesectoderm, and to prevent the ectopic expression of sim dorsally in the neuroectoderm. We have shown that repression of simtranscription by Su(H) is direct and independent of Notchactivity. Conversely, activation of sim transcription by Notch requires the Su(H)-binding sites. Thus, Notch signalling appears to relieve the repression exerted by Su(H) and to up-regulate simtranscription in the mesectoderm. We propose a model in which repression by Su(H) and derepression by Notch are essential to allow for the definition of a single row of mesectodermal cells in the blastoderm embryo.

Bibliography

Morel, V., & Schweisguth, F. (2000). Repression by Suppressor of Hairless and activation by Notch are required to define a single row of single-minded expressing cells in the Drosophila embryo. Genes & Development, 14(3), 377â388.

Authors 2

Véronique Morel (first)
François Schweisguth (additional)

References 44 Referenced 179

10.1126/science.284.5415.770
10.1101/gad.9.21.2609
10.1101/gad.8.20.2491
Campos-Ortega J.A. Hartenstein V. (1997) The embryonic development of Drosophila melanogaster. (Springer, Berlin, Germany). (10.1007/978-3-662-22489-2)
10.1093/genetics/144.4.1673 / Genetics / The autosomal FLP-DFS technique for generating germ-line mosaics in Drosophila melanogaster. by Chou (1996)
10.1101/gad.12.5.607
10.1016/0092-8674(88)90538-7
{'key': '2021111419181288000_14.3.377.8', 'first-page': '3310', 'article-title': 'The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.', 'volume': '14', 'author': 'Dou', 'year': '1994', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor. by Dou (1994)
10.1083/jcb.113.3.657
10.1101/gad.12.2.274
10.1126/science.8016657
10.1128/MCB.16.3.952 / Mol. Cell. Biol. / Truncated mammalian Notch1 activates CBF1/RBPJk-repressed genes by a mechanism resembling that of Epstein-Barr virus EBNA2. by Hsieh (1996)
10.1073/pnas.96.1.23
10.1101/gad.6.8.1518
10.1038/377355a0
10.1101/gad.12.15.2269
10.1073/pnas.89.8.3414
{'key': '2021111419181288000_14.3.377.18', 'first-page': '171', 'article-title': 'Specification of the Drosophila CNS midline cell lineage: Direct control of single-minded transcription by dorsal/ventral patterning genes.', 'volume': '7', 'author': 'Kasai', 'year': '1998', 'journal-title': 'Gene Expr.'} / Gene Expr. / Specification of the Drosophila CNS midline cell lineage: Direct control of single-minded transcription by dorsal/ventral patterning genes. by Kasai (1998)
10.1101/gad.12.23.3728
10.1016/0092-8674(91)90509-W
10.1242/dev.117.2.493 / Development / Implications of dynamic patterns of Delta and Notch expression for cellular interactions during Drosophila development. by Kooh (1993)
10.1126/science.1925551
10.1073/pnas.82.2.488
10.1101/gad.9.21.2598
10.1016/S0960-9822(98)70300-8
10.1101/gad.5.9.1568
10.1242/dev.120.4.853 / Development / Autonomy and non-autonomy in Drosophila mesoderm determination and morphogenesis. by Leptin (1994)
10.1101/gad.7.10.1949
10.1242/dev.121.1.219 / Development / Neurogenic genes control gene expression at the transcriptional level in early neurogenesis and in mesectoderm specification. by Martin-Bermudo (1995)
10.1242/dev.120.1.123 / Development / The formation of commissures in the Drosophila CNS depends on the midline cells and on the Notch gene. by Menne (1994)
10.1016/0925-4773(93)90043-W
10.1016/0092-8674(90)90288-P
10.1016/0092-8674(91)90292-7
10.1006/dbio.1999.9324
10.1016/S0959-437X(96)80062-1
10.1038/30756
10.1016/0092-8674(92)90641-O
10.1016/0092-8674(92)90466-P
10.1016/S0092-8674(00)81193-9
10.1016/0092-8674(88)90537-5
10.1016/0378-1119(88)90177-1
10.1093/nar/22.6.965
10.1242/dev.120.12.3563 / Development / Control of CNS midline transcription by asymmetric E-box-like elements: Similarity to xenobiotic responsive regulation. by Wharton (1994)
10.1016/S0960-9822(99)80211-5

Dates

Type	When
Created	3 years, 9 months ago (Nov. 14, 2021, 10:27 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:28 p.m.)
Indexed	11 months ago (Sept. 23, 2024, 12:27 a.m.)
Issued	25 years, 6 months ago (Feb. 1, 2000)
Published	25 years, 6 months ago (Feb. 1, 2000)
Published Online	25 years, 6 months ago (Feb. 1, 2000)
Published Print	25 years, 6 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{Morel_2000, title={Repression by Suppressor of Hairless and activation by Notch are required to define a single row of single-minded expressing cells in the Drosophila embryo}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.14.3.377}, DOI={10.1101/gad.14.3.377}, number={3}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Morel, Véronique and Schweisguth, François}, year={2000}, month=feb, pages={377–388} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        23
      ]
    ],
    "date-time": "2024-09-23T04:27:21Z",
    "timestamp": 1727065641017
  },
  "reference-count": 44,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "3",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        2,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Notch signal transduction appears to involve the ligand-induced intracellular processing of Notch, and the formation of a processed Notch-Suppressor of Hairless complex that binds DNA and activates the transcription of Notch target genes. This suggests that loss of either<jats:italic>Notch</jats:italic> or <jats:italic>Su(H)</jats:italic> activities should lead to similar cell fate changes. However, previous data indicate that, in the<jats:italic>Drosophila</jats:italic> blastoderm embryo, mesectoderm specification requires <jats:italic>Notch</jats:italic> but not <jats:italic>Su(H)</jats:italic> activity. The determination of the mesectodermal fate is specified by Single-minded (Sim), a transcription factor expressed in a single row of cells abutting the mesoderm. The molecular mechanisms by which the dorsoventral gradient of nuclear Dorsal establishes the single-cell wide territory of <jats:italic>sim</jats:italic> expression are not fully understood. We have found that <jats:italic>Notch</jats:italic> activity is required for <jats:italic>sim</jats:italic>expression in cellularizing embryos. In contrast, at this stage,<jats:italic>Su(H)</jats:italic> has a dual function. <jats:italic>Su(H)</jats:italic> activity was required to up-regulate <jats:italic>sim</jats:italic> expression in the mesectoderm, and to prevent the ectopic expression of <jats:italic>sim</jats:italic> dorsally in the neuroectoderm. We have shown that repression of <jats:italic>sim</jats:italic>transcription by Su(H) is direct and independent of <jats:italic>Notch</jats:italic>activity. Conversely, activation of <jats:italic>sim</jats:italic> transcription by Notch requires the Su(H)-binding sites. Thus, Notch signalling appears to relieve the repression exerted by Su(H) and to up-regulate <jats:italic>sim</jats:italic>transcription in the mesectoderm. We propose a model in which repression by Su(H) and derepression by Notch are essential to allow for the definition of a single row of mesectodermal cells in the blastoderm embryo.</jats:p>",
  "DOI": "10.1101/gad.14.3.377",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T03:27:54Z",
    "timestamp": 1636946874000
  },
  "page": "377-388",
  "source": "Crossref",
  "is-referenced-by-count": 179,
  "title": "Repression by Suppressor of Hairless and activation by Notch are required to define a single row of <i>single-minded</i> expressing cells in the <i>Drosophila</i> embryo",
  "prefix": "10.1101",
  "volume": "14",
  "author": [
    {
      "given": "V\u00e9ronique",
      "family": "Morel",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Fran\u00e7ois",
      "family": "Schweisguth",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        2000,
        2,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111419181288000_14.3.377.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.284.5415.770"
    },
    {
      "key": "2021111419181288000_14.3.377.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.21.2609"
    },
    {
      "key": "2021111419181288000_14.3.377.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.20.2491"
    },
    {
      "key": "2021111419181288000_14.3.377.4",
      "doi-asserted-by": "crossref",
      "unstructured": "Campos-Ortega J.A. Hartenstein V. (1997) The embryonic development of Drosophila melanogaster. (Springer, Berlin, Germany).",
      "DOI": "10.1007/978-3-662-22489-2"
    },
    {
      "key": "2021111419181288000_14.3.377.5",
      "doi-asserted-by": "crossref",
      "first-page": "1673",
      "DOI": "10.1093/genetics/144.4.1673",
      "article-title": "The autosomal FLP-DFS technique for generating germ-line mosaics in Drosophila melanogaster.",
      "volume": "144",
      "author": "Chou",
      "year": "1996",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111419181288000_14.3.377.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.5.607"
    },
    {
      "key": "2021111419181288000_14.3.377.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(88)90538-7"
    },
    {
      "key": "2021111419181288000_14.3.377.8",
      "first-page": "3310",
      "article-title": "The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.",
      "volume": "14",
      "author": "Dou",
      "year": "1994",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111419181288000_14.3.377.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.113.3.657"
    },
    {
      "key": "2021111419181288000_14.3.377.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.2.274"
    },
    {
      "key": "2021111419181288000_14.3.377.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8016657"
    },
    {
      "key": "2021111419181288000_14.3.377.12",
      "doi-asserted-by": "crossref",
      "first-page": "952",
      "DOI": "10.1128/MCB.16.3.952",
      "article-title": "Truncated mammalian Notch1 activates CBF1/RBPJk-repressed genes by a mechanism resembling that of Epstein-Barr virus EBNA2.",
      "volume": "16",
      "author": "Hsieh",
      "year": "1996",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111419181288000_14.3.377.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.1.23"
    },
    {
      "key": "2021111419181288000_14.3.377.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.8.1518"
    },
    {
      "key": "2021111419181288000_14.3.377.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377355a0"
    },
    {
      "key": "2021111419181288000_14.3.377.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.15.2269"
    },
    {
      "key": "2021111419181288000_14.3.377.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.8.3414"
    },
    {
      "key": "2021111419181288000_14.3.377.18",
      "first-page": "171",
      "article-title": "Specification of the Drosophila CNS midline cell lineage: Direct control of single-minded transcription by dorsal/ventral patterning genes.",
      "volume": "7",
      "author": "Kasai",
      "year": "1998",
      "journal-title": "Gene Expr."
    },
    {
      "key": "2021111419181288000_14.3.377.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.23.3728"
    },
    {
      "key": "2021111419181288000_14.3.377.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90509-W"
    },
    {
      "key": "2021111419181288000_14.3.377.21",
      "doi-asserted-by": "crossref",
      "first-page": "493",
      "DOI": "10.1242/dev.117.2.493",
      "article-title": "Implications of dynamic patterns of Delta and Notch expression for cellular interactions during Drosophila development.",
      "volume": "117",
      "author": "Kooh",
      "year": "1993",
      "journal-title": "Development"
    },
    {
      "key": "2021111419181288000_14.3.377.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1925551"
    },
    {
      "key": "2021111419181288000_14.3.377.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.2.488"
    },
    {
      "key": "2021111419181288000_14.3.377.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.21.2598"
    },
    {
      "key": "2021111419181288000_14.3.377.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(98)70300-8"
    },
    {
      "key": "2021111419181288000_14.3.377.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.5.9.1568"
    },
    {
      "key": "2021111419181288000_14.3.377.27",
      "doi-asserted-by": "crossref",
      "first-page": "853",
      "DOI": "10.1242/dev.120.4.853",
      "article-title": "Autonomy and non-autonomy in Drosophila mesoderm determination and morphogenesis.",
      "volume": "120",
      "author": "Leptin",
      "year": "1994",
      "journal-title": "Development"
    },
    {
      "key": "2021111419181288000_14.3.377.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.10.1949"
    },
    {
      "key": "2021111419181288000_14.3.377.29",
      "doi-asserted-by": "crossref",
      "first-page": "219",
      "DOI": "10.1242/dev.121.1.219",
      "article-title": "Neurogenic genes control gene expression at the transcriptional level in early neurogenesis and in mesectoderm specification.",
      "volume": "121",
      "author": "Martin-Bermudo",
      "year": "1995",
      "journal-title": "Development"
    },
    {
      "key": "2021111419181288000_14.3.377.30",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1242/dev.120.1.123",
      "article-title": "The formation of commissures in the Drosophila CNS depends on the midline cells and on the Notch gene.",
      "volume": "120",
      "author": "Menne",
      "year": "1994",
      "journal-title": "Development"
    },
    {
      "key": "2021111419181288000_14.3.377.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0925-4773(93)90043-W"
    },
    {
      "key": "2021111419181288000_14.3.377.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90288-P"
    },
    {
      "key": "2021111419181288000_14.3.377.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90292-7"
    },
    {
      "key": "2021111419181288000_14.3.377.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1999.9324"
    },
    {
      "key": "2021111419181288000_14.3.377.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-437X(96)80062-1"
    },
    {
      "key": "2021111419181288000_14.3.377.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/30756"
    },
    {
      "key": "2021111419181288000_14.3.377.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90641-O"
    },
    {
      "key": "2021111419181288000_14.3.377.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90466-P"
    },
    {
      "key": "2021111419181288000_14.3.377.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81193-9"
    },
    {
      "key": "2021111419181288000_14.3.377.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(88)90537-5"
    },
    {
      "key": "2021111419181288000_14.3.377.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(88)90177-1"
    },
    {
      "key": "2021111419181288000_14.3.377.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/22.6.965"
    },
    {
      "key": "2021111419181288000_14.3.377.43",
      "doi-asserted-by": "crossref",
      "first-page": "3563",
      "DOI": "10.1242/dev.120.12.3563",
      "article-title": "Control of CNS midline transcription by asymmetric E-box-like elements: Similarity to xenobiotic responsive regulation.",
      "volume": "120",
      "author": "Wharton",
      "year": "1994",
      "journal-title": "Development"
    },
    {
      "key": "2021111419181288000_14.3.377.44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(99)80211-5"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.14.3.377",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T03:28:10Z",
    "timestamp": 1636946890000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.14.3.377"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        2,
        1
      ]
    ]
  },
  "references-count": 44,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2000,
          2,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.14.3.377"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.14.3.377",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2000,
        2,
        1
      ]
    ]
  }
}