DOI: 10.1101/gad.14.13.1631. Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro

Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro

10.1101/gad.14.13.1631

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

An interaction between the origin recognition complex (ORC) and Cdc6p is the first and a key step in the initiation of chromosomal DNA replication. We describe the assembly of an origin-dependent complex containing ORC and Cdc6p from Saccharomyces cerevisiae. Cdc6p increases the DNA binding specificity of ORC by inhibiting non-specific DNA binding of ORC. Cdc6p induces a concomitant change in the conformation of ORC and mutations in the Cdc6p Walker A and Walker B motifs, or ATP-γ-S inhibited these activities of Cdc6p. These data suggest that Cdc6p modifies ORC function at DNA replication origins. On the basis of these results in yeast, we propose that Cdc6p may be an essential determinant of origin specificity in metazoan species.

Bibliography

Mizushima, T., Takahashi, N., & Stillman, B. (2000). Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro. Genes & Development, 14(13), 1631â1641.

Authors 3

Tohru Mizushima (first)
Naoko Takahashi (additional)
Bruce Stillman (additional)

References 51 Referenced 89

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Dates

Type	When
Created	3 years, 9 months ago (Nov. 14, 2021, 10:33 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:33 p.m.)
Indexed	11 months, 1 week ago (Sept. 13, 2024, 12:43 p.m.)
Issued	25 years, 1 month ago (July 1, 2000)
Published	25 years, 1 month ago (July 1, 2000)
Published Online	25 years, 1 month ago (July 1, 2000)
Published Print	25 years, 1 month ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Mizushima_2000, title={Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.14.13.1631}, DOI={10.1101/gad.14.13.1631}, number={13}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Mizushima, Tohru and Takahashi, Naoko and Stillman, Bruce}, year={2000}, month=jul, pages={1631–1641} }

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