DOI: 10.1101/gad.14.12.1439. Aberrant cell cycle checkpoint function and early embryonic death in <i>Chk1</i><sup>−/−</sup> mice

Aberrant cell cycle checkpoint function and early embryonic death in Chk1−/− mice

10.1101/gad.14.12.1439

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The recent discovery of checkpoint kinases has suggested the conservation of checkpoint mechanisms between yeast and mammals. In yeast, the protein kinase Chk1 is thought to mediate signaling associated with the DNA damage checkpoint of the cell cycle. However, the function of Chk1 in mammals has remained unknown. Targeted disruption of Chk1 in mice showed thatChk1−/− embryos exhibit gross morphologic abnormalities in nuclei as early as the blastocyst stage. In culture, Chk1−/− blastocysts showed a severe defect in outgrowth of the inner cell mass and died of apoptosis. DNA replication block and DNA damage failed to arrest the cell cycle before initiation of mitosis inChk1−/− embryos. These results may indicate that Chk1 is indispensable for cell proliferation and survival through maintaining the G2 checkpoint in mammals.

Bibliography

Takai, H., Tominaga, K., Motoyama, N., Minamishima, Y. A., Nagahama, H., Tsukiyama, T., Ikeda, K., Nakayama, K., Nakanishi, M., & Nakayama, K. (2000). Aberrant cell cycle checkpoint function and early embryonic death in Chk1â/â mice. Genes & Development, 14(12), 1439â1447.

Authors 10

Hiroyuki Takai (first)
Kaoru Tominaga (additional)
Noboru Motoyama (additional)
Yohji A. Minamishima (additional)
Hiroyasu Nagahama (additional)
Tadasuke Tsukiyama (additional)
Kyoji Ikeda (additional)
Keiko Nakayama (additional)
Makoto Nakanishi (additional)
Kei-ichi Nakayama (additional)

References 38 Referenced 383

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{'key': '2021111419211507000_14.12.1439.28', 'first-page': '4375', 'article-title': 'Inhibition of ATM and ATR kinase activities by the radiosensitizing agent, caffeine.', 'volume': '59', 'author': 'Sarkaria', 'year': '1999', 'journal-title': 'Cancer Res.'} / Cancer Res. / Inhibition of ATM and ATR kinase activities by the radiosensitizing agent, caffeine. by Sarkaria (1999)
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Dates

Type	When
Created	3 years, 9 months ago (Nov. 14, 2021, 10:32 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:33 p.m.)
Indexed	3 weeks, 5 days ago (July 27, 2025, 3:45 a.m.)
Issued	25 years, 2 months ago (June 15, 2000)
Published	25 years, 2 months ago (June 15, 2000)
Published Online	25 years, 2 months ago (June 15, 2000)
Published Print	25 years, 2 months ago (June 15, 2000)

Funders 0

None

BibTeX

@article{Takai_2000, title={Aberrant cell cycle checkpoint function and early embryonic death in Chk1−/− mice}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.14.12.1439}, DOI={10.1101/gad.14.12.1439}, number={12}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Takai, Hiroyuki and Tominaga, Kaoru and Motoyama, Noboru and Minamishima, Yohji A. and Nagahama, Hiroyasu and Tsukiyama, Tadasuke and Ikeda, Kyoji and Nakayama, Keiko and Nakanishi, Makoto and Nakayama, Kei-ichi}, year={2000}, month=jun, pages={1439–1447} }

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