DOI: 10.1101/gad.14.10.1229. Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast

Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast

10.1101/gad.14.10.1229

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The MAP kinase Hog1 transiently accumulates in the nucleus upon activation. Although Hog1 nuclear export correlates with its dephosphorylation, we find that dephosphorylation is not necessary for export. Unexpectedly, a strain lacking the nuclear protein tyrosine phosphatase, Ptp2, showed decreased Hog1 nuclear retention, while a strain lacking the cytoplasmic Ptp3 showed prolonged Hog1 nuclear accumulation, consistent with Ptp2 being a nuclear tether for Hog1 and Ptp3 being a cytoplasmic anchor. In support of this result PTP2overexpression sequestered Hog1 in the nucleus while PTP3overexpression restricted Hog1 to the cytoplasm. Thus, Ptp2 and Ptp3 regulate Hog1 localization by binding Hog1.

Bibliography

Mattison, C. P., & Ota, I. M. (2000). Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast. Genes & Development, 14(10), 1229â1235.

Authors 2

Christopher P. Mattison (first)
Irene M. Ota (additional)

References 34 Referenced 105

10.1016/S0960-9822(98)70442-7
10.1002/j.1460-2075.1994.tb06426.x / EMBO J. / The Drosophila rolled locus encodes a MAP kinase required in the sevenless signal transduction pathway. by Biggs (1994)
10.1016/0014-5793(94)00958-9
10.1126/science.7681220
10.1093/emboj/18.3.664
10.1016/0092-8674(94)90362-X
{'key': '2021111419203944000_14.10.1229.7', 'first-page': '915', 'article-title': 'Nuclear localization and regulation of erk- and rsk-encoded protein kinases.', 'volume': '12', 'author': 'Chen', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Nuclear localization and regulation of erk- and rsk-encoded protein kinases. by Chen (1992)
10.1093/emboj/17.19.5606
10.1093/emboj/16.8.1901
10.1091/mbc.10.5.1395
10.1101/gad.12.10.1464
10.1016/0378-1119(88)90185-0
10.1083/jcb.122.5.1089
Guthrie, C. and R.G. Fink. 1991. Guide to yeast genetics and molecular biology. Methods Enzymol. 194..
10.1016/0378-1119(89)90403-4
10.1038/386296a0
10.1074/jbc.272.28.17749
10.1016/S0092-8674(00)81189-7
10.1083/jcb.122.5.1079
10.1083/jcb.142.3.625
{'key': '2021111419203944000_14.10.1229.21', 'first-page': '5408', 'article-title': 'Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae.', 'volume': '13', 'author': 'Maeda', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. by Maeda (1993)
10.1038/369242a0
10.1128/MCB.19.11.7651 / Mol. Cell. Biol. / Differential Regulation of the cell wall integrity pathway in budding yeast by the protein tyrosine phosphatases, Ptp2 and Ptp3. by Mattison (1999)
10.1101/gad.12.18.2874
10.1073/pnas.89.6.2355
10.1091/mbc.10.4.1147
10.1002/j.1460-2075.1994.tb06758.x / EMBO J. / The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. by Schuller (1994)
10.1042/bj2880351 / Biochem. J. / Sustained activation of the mitogen-activated protein (MAP) kinase cascade may be required for differentiation of PC12 cells. Comparison of the effects of nerve growth factor and epidermal growth factor. by Traverse (1992)
10.1128/MCB.16.2.704 / Mol. Cell. Biol. / Schizosaccharomyces pombe pcr1+ encodes a CREB/ATF protein involved in regulation of gene expression for sexual development. by Watanabe (1996)
10.1128/MCB.17.3.1289 / Mol. Cell. Biol. / Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases. by Wurgler-Murphy (1997)
10.1038/33451
10.1101/gad.11.13.1690
10.1074/jbc.270.21.12665
10.1074/jbc.274.31.21900

Dates

Type	When
Created	3 years, 9 months ago (Nov. 14, 2021, 10:31 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:32 p.m.)
Indexed	1 month ago (July 23, 2025, 8:38 a.m.)
Issued	25 years, 3 months ago (May 15, 2000)
Published	25 years, 3 months ago (May 15, 2000)
Published Online	25 years, 3 months ago (May 15, 2000)
Published Print	25 years, 3 months ago (May 15, 2000)

Funders 0

None

BibTeX

@article{Mattison_2000, title={Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.14.10.1229}, DOI={10.1101/gad.14.10.1229}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Mattison, Christopher P. and Ota, Irene M.}, year={2000}, month=may, pages={1229–1235} }

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