DOI: 10.1101/gad.14.1.55. Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression

Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression

10.1101/gad.14.1.55

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The transcriptional corepressor SMRT functions by mediating the repressive effect of transcription factors involved in diverse signaling pathways. The mechanism by which SMRT represses basal transcription has been proposed to involve the indirect recruitment of histone deacetylase HDAC1 via the adaptor mSin3A. In contrast to this model, a two-hybrid screen on SMRT-interacting proteins resulted in the isolation of the recently described HDAC5 and a new family member termed HDAC7. Molecular and biochemical results indicate that this interaction is direct and in vivo evidence colocalizes SMRT, mHDAC5, and mHDAC7 to a distinct nuclear compartment. Surprisingly, HDAC7 can interact with mSin3A in yeast and in mammalian cells, suggesting association of multiple repression complexes. Taken together, our results provide the first evidence that SMRT-mediated repression is promoted by class I and class II histone deacetylases and that SMRT can recruit class II histone deacetylases in a mSin3A-independent fashion.

Bibliography

Kao, H.-Y., Downes, M., Ordentlich, P., & Evans, R. M. (2000). Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression. Genes & Development, 14(1), 55â66.

Authors 4

Hung-Ying Kao (first)
Michael Downes (additional)
Peter Ordentlich (additional)
Ronald M. Evans (additional)

References 52 Referenced 333

10.1038/387049a0
10.1128/MCB.19.12.8219 / Mol. Cell. Biol. / Pbx/Hox heterodimers recruit co-activator/co-repressor complexes in an isoform-specific manner. by Asahara (1999)
10.1210/me.13.7.1155
10.1038/384641a0
10.1101/gad.12.11.1638
10.1074/jbc.271.26.15837
10.1016/S0092-8674(00)80516-4
10.1038/377454a0
10.1073/pnas.94.20.10762
10.1128/MCB.19.8.5504 / Mol. Cell. Biol. / Histone deacetylase 1 can repress transcription by binding to Sp1. by Doetzlhofer (1999)
10.1128/MCB.19.5.3383 / Mol. Cell. Biol. / Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily. by Dressel (1999)
10.1073/pnas.95.6.2795
10.1074/jbc.274.17.11713
10.1073/pnas.96.9.4868
10.1016/S0092-8674(00)80214-7
10.1038/ng0298-126
10.1038/387043a0
10.1073/pnas.93.10.4948
10.1073/pnas.94.17.9028
10.1038/377397a0
10.1038/sj.onc.1202197
10.1128/MCB.19.1.916 / Mol. Cell. Biol. / E2F and histone deacetylase mediate transforming growth factor beta repression of cdc25A during keratinocyte cell cycle arrest. by Iavarone (1999)
10.1128/MCB.18.9.5121 / Mol. Cell. Biol. / Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. by Kadosh (1998)
10.1016/S0092-8674(00)81118-6
10.1101/gad.12.15.2269
10.1016/S0092-8674(00)80215-9
10.1016/S1097-2765(00)80111-2
10.1038/35895
10.1016/0092-8674(95)90200-7
10.1016/0092-8674(95)90199-X
10.1093/emboj/18.18.5099
10.1093/emboj/17.19.5734
10.1016/S0092-8674(00)80218-4
10.1038/30764
10.1016/S0092-8674(00)82001-2
10.1126/science.270.5240.1354
10.1073/pnas.96.6.2639
10.1128/JVI.73.7.5688-5697.1999 / J. Virol. / Epstein-Barr virus nuclear antigen 3C interacts with histone deacetylase to repress transcription. by Radkov (1999)
10.1073/pnas.93.25.14503
10.1038/33952
10.1038/38304
10.1038/42652
10.1074/jbc.274.4.2440
10.1101/sqb.1998.63.435
10.1074/jbc.273.42.27695
10.1016/S1097-2765(00)80299-3
10.1073/pnas.93.23.12845
10.1038/382319a0
10.1073/pnas.93.20.10626
10.1128/MCB.16.10.5458 / Mol. Cell. Biol. / A nuclear hormone receptor corepressor mediates transcriptional silencing by receptors with distinct repression domains. by Zamir (1996)
10.1016/S0092-8674(00)80216-0
10.1016/S1097-2765(00)80102-1

Dates

Type	When
Created	3 years, 9 months ago (Nov. 14, 2021, 10:27 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:30 p.m.)
Indexed	6 days, 8 hours ago (Aug. 22, 2025, 1:07 a.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	25 years, 7 months ago (Jan. 1, 2000)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Kao_2000, title={Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.14.1.55}, DOI={10.1101/gad.14.1.55}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kao, Hung-Ying and Downes, Michael and Ordentlich, Peter and Evans, Ronald M.}, year={2000}, month=jan, pages={55–66} }

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