DOI: 10.1101/gad.1352305. Polycomb mediates Myc autorepression and its transcriptional control of many loci in <i>Drosophila</i>

Polycomb mediates Myc autorepression and its transcriptional control of many loci in Drosophila

10.1101/gad.1352305

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Aberrant accumulation of the Myc oncoprotein propels proliferation and induces carcinogenesis. In normal cells, however, an abundance of Myc protein represses transcription at the c-myc locus. Cancer cells often lose this autorepression. We examined the control of myc in Drosophila and show here that the Drosophila ortholog, dmyc, also undergoes autorepression. We find that the developmental repressor Polycomb (Pc) is required for dmyc autorepression, and that this Pc-dMyc-mediated repression spreads across an 875-kb region encompassing the dmyc gene. To further investigate the relationship between Myc and Polycomb, we used microarrays to identify genes regulated by each, and identify a striking relationship between the two: A large set of dMyc activation targets is normally repressed by Pc, and 73% of dMyc repression targets require Pc for this repression. Chromatin immunoprecipitation confirmed that many dMyc-Pc-repressed loci have an epigenetic mark recognized by Pc. Our results suggest a novel relationship between Myc and Polycomb, wherein Myc enhances Polycomb repression in order to repress targets, and Myc suppresses Polycomb repression in order to activate targets.

Bibliography

Goodliffe, J. M., Wieschaus, E., & Cole, M. D. (2005). Polycomb mediates Myc autorepression and its transcriptional control of many loci in Drosophila. Genes & Development, 19(24), 2941â2946.

Authors 3

Julie M. Goodliffe (first)
Eric Wieschaus (additional)
Michael D. Cole (additional)

References 26 Referenced 36

Dates

Type	When
Created	19 years, 8 months ago (Dec. 15, 2005, 3:52 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 11:43 a.m.)
Indexed	1 year, 2 months ago (June 12, 2024, 10:22 p.m.)
Issued	19 years, 8 months ago (Dec. 15, 2005)
Published	19 years, 8 months ago (Dec. 15, 2005)
Published Online	19 years, 8 months ago (Dec. 15, 2005)
Published Print	19 years, 8 months ago (Dec. 15, 2005)

Funders 0

None

BibTeX

@article{Goodliffe_2005, title={Polycomb mediates Myc autorepression and its transcriptional control of many loci in Drosophila}, volume={19}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1352305}, DOI={10.1101/gad.1352305}, number={24}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Goodliffe, Julie M. and Wieschaus, Eric and Cole, Michael D.}, year={2005}, month=dec, pages={2941–2946} }

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