DOI: 10.1101/gad.1314205. Global analysis of positive and negative pre-mRNA splicing regulators in <i>Drosophila</i>

Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila

10.1101/gad.1314205

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

To gain insight into splicing regulation, we developed a microarray to assay all annotated alternative splicing events in Drosophila melanogaster and identified the alternative splice events controlled by four splicing regulators: dASF/SF2, B52/SRp55, hrp48, and PSI. The number of events controlled by each of these factors was found to be highly variable: dASF/SF2 strongly affects >300 splicing events, whereas PSI strongly affects only 43 events. Pairwise analysis also revealed many instances of splice site usage affected by multiple factors and provides the framework to understand the network controlling the alternatively spliced mRNA isoforms that compose the Drosophila transcriptome.

Bibliography

Blanchette, M., Green, R. E., Brenner, S. E., & Rio, D. C. (2005). Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila. Genes & Development, 19(11), 1306â1314.

Authors 4

Marco Blanchette (first)
Richard E. Green (additional)
Steven E. Brenner (additional)
Donald C. Rio (additional)

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Dates

Type	When
Created	20 years, 2 months ago (June 3, 2005, 1:53 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 10:30 a.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 4:26 a.m.)
Issued	20 years, 2 months ago (June 1, 2005)
Published	20 years, 2 months ago (June 1, 2005)
Published Online	20 years, 2 months ago (June 3, 2005)
Published Print	20 years, 2 months ago (June 1, 2005)

Funders 0

None

BibTeX

@article{Blanchette_2005, title={Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila}, volume={19}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1314205}, DOI={10.1101/gad.1314205}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Blanchette, Marco and Green, Richard E. and Brenner, Steven E. and Rio, Donald C.}, year={2005}, month=jun, pages={1306–1314} }

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