DOI: 10.1101/gad.1284005. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9

10.1101/gad.1284005

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin. GLP-deficiency led to embryonic lethality, a severe reduction of H3-K9 mono- and dimethylation, the induction of Mage-a gene expression, and HP1 relocalization in embryonic stem cells, all of which were phenotypes of G9a-deficiency. Furthermore, we show that G9a and GLP formed a stoichiometric heteromeric complex in a wide variety of cell types. Biochemical analyses revealed that formation of the G9a/GLP complex was dependent on their enzymatic SET domains. Taken together, our new findings revealed that G9a and GLP cooperatively exert H3-K9 methyltransferase function in vivo, likely through the formation of higher-order heteromeric complexes.

Bibliography

Tachibana, M., Ueda, J., Fukuda, M., Takeda, N., Ohta, T., Iwanari, H., Sakihama, T., Kodama, T., Hamakubo, T., & Shinkai, Y. (2005). Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes & Development, 19(7), 815â826.

Authors 10

Makoto Tachibana (first)
Jun Ueda (additional)
Mikiko Fukuda (additional)
Naoki Takeda (additional)
Tsutomu Ohta (additional)
Hiroko Iwanari (additional)
Toshiko Sakihama (additional)
Tatsuhiko Kodama (additional)
Takao Hamakubo (additional)
Yoichi Shinkai (additional)

References 40 Referenced 709

Dates

Type	When
Created	20 years, 5 months ago (March 17, 2005, 11:55 p.m.)
Deposited	3 years, 9 months ago (Nov. 16, 2021, 10:27 a.m.)
Indexed	8 hours, 27 minutes ago (Aug. 26, 2025, 2:57 a.m.)
Issued	20 years, 5 months ago (March 17, 2005)
Published	20 years, 5 months ago (March 17, 2005)
Published Online	20 years, 5 months ago (March 17, 2005)
Published Print	20 years, 4 months ago (April 1, 2005)

Funders 0

None

BibTeX

@article{Tachibana_2005, title={Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9}, volume={19}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1284005}, DOI={10.1101/gad.1284005}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Tachibana, Makoto and Ueda, Jun and Fukuda, Mikiko and Takeda, Naoki and Ohta, Tsutomu and Iwanari, Hiroko and Sakihama, Toshiko and Kodama, Tatsuhiko and Hamakubo, Takao and Shinkai, Yoichi}, year={2005}, month=mar, pages={815–826} }

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