DOI: 10.1101/gad.12.23.3728. Ligand-induced cleavage and regulation of nuclear entry of Notch in <i>Drosophila melanogaster</i> embryos

Ligand-induced cleavage and regulation of nuclear entry of Notch in Drosophila melanogaster embryos

10.1101/gad.12.23.3728

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Notch, a transmembrane protein found in a wide range of organisms, is a component of a pathway that mediates cell-fate decisions that involve intercellular communication. In this paper, we show that inDrosophila melanogaster, Notch (N) is processed in a ligand-dependent fashion to generate phosphorylated, soluble intracellular derivatives. Suppressor of Hairless [Su(H)] is predominantly associated with soluble intracellular N. It has been demonstrated by others that N has access to the nucleus, and we show that when tethered directly to DNA, the cytoplasmic domain of N can activate transcription. Conversely, a viral activator fused to Su(H) can substitute for at least some N functions during embryogenesis. We suggest that one function of soluble forms of N is to bind to Su(H), and in the nucleus, to act directly as a transcriptional transactivator of the latter protein. Although N has functional nuclear localization signals, the N/Su(H) complex accumulates in the cytoplasm and on membranes suggesting that its nuclear entry is regulated. Localization studies in cultured cells and embryos suggest that Su(H) plays a role in this regulation, with the relative levels of Delta, N and Su(H) determining whether a N/Su(H) complex enters the nucleus.

Bibliography

Kidd, S., Lieber, T., & Young, M. W. (1998). Ligand-induced cleavage and regulation of nuclear entry of Notch in Drosophila melanogaster embryos. Genes & Development, 12(23), 3728â3740.

Authors 3

Simon Kidd (first)
Toby Lieber (additional)
Michael W. Young (additional)

References 60 Referenced 196

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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:26 p.m.)
Indexed	2 months, 2 weeks ago (June 10, 2025, 2:06 p.m.)
Issued	26 years, 8 months ago (Dec. 1, 1998)
Published	26 years, 8 months ago (Dec. 1, 1998)
Published Online	26 years, 8 months ago (Dec. 1, 1998)
Published Print	26 years, 8 months ago (Dec. 1, 1998)

Funders 0

None

BibTeX

@article{Kidd_1998, title={Ligand-induced cleavage and regulation of nuclear entry of Notch in Drosophila melanogaster embryos}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.23.3728}, DOI={10.1101/gad.12.23.3728}, number={23}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kidd, Simon and Lieber, Toby and Young, Michael W.}, year={1998}, month=dec, pages={3728–3740} }

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