DOI: 10.1101/gad.12.18.2842. Mitotic inactivation of a human SWI/SNF chromatin remodeling complex

Mitotic inactivation of a human SWI/SNF chromatin remodeling complex

10.1101/gad.12.18.2842

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and hBrm, which are components of human SWI/SNF (hSWI/SNF) complexes, were recently shown to be phosphorylated during mitosis. This suggested that phosphorylation might be used as a switch to modulate SWI/SNF activity. Using an epitope-tag strategy, we have purified hSWI/SNF complexes at different stages of the cell cycle, and found that hSWI/SNF was inactive in cells blocked in G2–M. Mitotic hSWI/SNF contained Brg1 but not hBrm, and was phosphorylated on at least two subunits, hSWI3 and Brg1. In vitro, active hSWI/SNF from asynchronous cells can be phosphorylated and inactivated by ERK1, and reactivated by dephosphorylation. hSWI/SNF isolated as cells traversed mitosis regained activity when its subunits were dephosphorylated either in vitro or in vivo. We propose that this transitional inactivation and reactivation of hSWI/SNF is required for formation of a repressed chromatin structure during mitosis and reformation of an active chromatin structure as cells leave mitosis.

Bibliography

Sif, S., Stukenberg, P. T., Kirschner, M. W., & Kingston, R. E. (1998). Mitotic inactivation of a human SWI/SNF chromatin remodeling complex. Genes & Development, 12(18), 2842â2851.

Authors 4

Saïd Sif (first)
P. Todd Stukenberg (additional)
Marc W. Kirschner (additional)
Robert E. Kingston (additional)

References 48 Referenced 225

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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:52 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:20 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:33 p.m.)
Issued	26 years, 11 months ago (Sept. 15, 1998)
Published	26 years, 11 months ago (Sept. 15, 1998)
Published Online	26 years, 11 months ago (Sept. 15, 1998)
Published Print	26 years, 11 months ago (Sept. 15, 1998)

Funders 0

None

BibTeX

@article{Sif_1998, title={Mitotic inactivation of a human SWI/SNF chromatin remodeling complex}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.18.2842}, DOI={10.1101/gad.12.18.2842}, number={18}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Sif, Saïd and Stukenberg, P. Todd and Kirschner, Marc W. and Kingston, Robert E.}, year={1998}, month=sep, pages={2842–2851} }

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