CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint
10.1101/gad.12.17.2698
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Initiation of DNA replication during the mitotic cell cycle requires the activation of a cyclin-dependent protein kinase (CDK). The B-type cyclins Clb5 and Clb6 are the primary activators of the S phase function of the budding yeast CDK Cdc28. However, in mitotically growing cells this role can be fulfilled by the other B-type cyclins Clb1–Clb4. We report here that cells undergoing meiotic development also require Clb dependent CDK activity for DNA replication. Diploidclb5/clb5 clb6/clb6 mutants are unable to perform premeiotic DNA replication. Despite this defect, the mutant cells progress into the meiotic program and undergo lethal segregation of unreplicated DNA suggesting that they fail to activate a checkpoint that restrains meiotic M phase until DNA replication is complete. We have found that a DNA replication checkpoint dependent on the ATM homolog MEC1 operates in wild-type cells during meiosis and can be invoked in response to inhibition of DNA synthesis. Although cells that lack clb5 and clb6 are unable to activate the meiotic DNA replication checkpoint, they do possess an intact DNA damage checkpoint which can restrain chromosome segregation in the face of DNA damage. We conclude that CLB5 and CLB6 are essential for premeiotic DNA replication and, consequently, for activation of a meiotic DNA replication checkpoint.

Bibliography

Stuart, D., & Wittenberg, C. (1998). CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint. Genes & Development, 12(17), 2698–2710.

Authors 2
  1. David Stuart (first)
  2. Curt Wittenberg (additional)
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Dates
Type When
Created 17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 10:21 p.m.)
Indexed 1 year, 1 month ago (July 10, 2024, 1:17 p.m.)
Issued 26 years, 11 months ago (Sept. 1, 1998)
Published 26 years, 11 months ago (Sept. 1, 1998)
Published Online 26 years, 11 months ago (Sept. 1, 1998)
Published Print 26 years, 11 months ago (Sept. 1, 1998)
Funders 0

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@article{Stuart_1998, title={CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.17.2698}, DOI={10.1101/gad.12.17.2698}, number={17}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Stuart, David and Wittenberg, Curt}, year={1998}, month=sep, pages={2698–2710} }