DOI: 10.1101/gad.12.17.2664. SNAP19 mediates the assembly of a functional core promoter complex (SNAP<sub>c</sub>) shared by RNA polymerases II and III

SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III

10.1101/gad.12.17.2664

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The basal transcription factor SNAPc binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAPc contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAPc. Here we reconstitute SNAPc from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAPc nucleates the assembly of two classes of initiation complexes.

Bibliography

Henry, R. W., Mittal, V., Ma, B., Kobayashi, R., & Hernandez, N. (1998). SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III. Genes & Development, 12(17), 2664â2672.

Authors 5

R. William Henry (first)
Vivek Mittal (additional)
Beicong Ma (additional)
Ryuji Kobayashi (additional)
Nouria Hernandez (additional)

References 23 Referenced 74

10.1128/MCB.16.10.5419 / Mol. Cell. Biol. / Cloning and characterization of the β subunit of human proximal sequence element-binding transcription factor and its involvement in transcription of small nuclear RNA genes by RNA polymerases II and III. by Bai (1996)
10.1126/science.270.5241.1506 / Science / Coactivator and promoter-selective properties of RNA polymerase I TAFs. by Beckmann (1995)
10.1016/0092-8674(94)90403-0
Dahlberg J.E. Lund E. (1988) The genes and transcription of the major small nuclear RNAs. in Structure and function of major and minor small nuclear ribonucleoprotein particles, ed Birnstiel M.L. (Springer Verlag, Berlin, Germany), pp 38–70. (10.1007/978-3-642-73020-7_2)
Geiduschek E.P. Kassavetis G.A. (1992) in RNA polymerase III transcription complexes. Transcriptional regulation, eds McKnight S.L. Yamamoto K.R. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY), pp 247–280.
10.1038/374653a0
10.1002/j.1460-2075.1996.tb01104.x / EMBO J. / Cloning and characterization of SNAP50, a subunit of the snRNA-activating protein complex SNAPc. by Henry (1996)
10.1073/pnas.92.21.9786
10.1038/227680a0
10.1016/0092-8674(89)90402-9
10.1101/gad.5.8.1477
10.1016/0092-8674(88)90029-3
10.1126/science.275.5303.1136
{'key': '2021111418563378000_12.17.2664.14', 'first-page': '3247', 'article-title': 'Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes.', 'volume': '12', 'author': 'Murphy', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes. by Murphy (1992)
10.1101/gad.10.21.2657
10.1074/jbc.271.25.14903
10.1002/j.1460-2075.1996.tb00545.x / EMBO J. / A suppressor of mutations in the class III transcription system encodes a component of yeast TFIIIB. by Rüth (1996)
10.1101/gad.7.8.1535
10.1073/pnas.93.9.4289
10.1002/j.1460-2075.1991.tb07711.x / EMBO J. / TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III. by Simmen (1991)
10.1128/MCB.18.1.368 / Mol. Cell. Biol. / The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1. by Wong (1998)
10.1016/S1569-2558(08)60061-2
10.1128/MCB.15.4.2019 / Mol. Cell. Biol. / Proximal sequence element-binding transcription factor (PTF) is a multisubunit complex required for transcription of both RNA polymerase II- and RNA polymerase III-dependent small nuclear RNA genes. by Yoon (1995)

Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:23 p.m.)
Indexed	2 months ago (June 19, 2025, 3:26 a.m.)
Issued	26 years, 11 months ago (Sept. 1, 1998)
Published	26 years, 11 months ago (Sept. 1, 1998)
Published Online	26 years, 11 months ago (Sept. 1, 1998)
Published Print	26 years, 11 months ago (Sept. 1, 1998)

Funders 0

None

BibTeX

@article{Henry_1998, title={SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.17.2664}, DOI={10.1101/gad.12.17.2664}, number={17}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Henry, R. William and Mittal, Vivek and Ma, Beicong and Kobayashi, Ryuji and Hernandez, Nouria}, year={1998}, month=sep, pages={2664–2672} }

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