DOI: 10.1101/gad.12.14.2188. DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D

DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D

10.1101/gad.12.14.2188

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

DNA-dependent protein kinase (DNA-PK), which is involved in DNA double-strand break repair and V(D)J recombination, is comprised of a DNA-targeting component termed Ku and an ∼465-kD catalytic subunit, DNA-PKcs. Although DNA-PK phosphorylates proteins in the presence of DSBs or other discontinuities in the DNA double helix in vitro, the possibility exists that it is also activated in other circumstances via its association with additional proteins. Here, through use of the yeast two-hybrid screen, we discover that the recently identified high affinity DNA binding protein C1D interacts with the putative leucine zipper region of DNA-PKcs. Furthermore, we show that C1D can interact with DNA-PK in mammalian cells and that C1D is a very effective DNA-PK substrate in vitro. Finally, we establish that C1D directs the activation of DNA-PK in a manner that does not require DNA termini. Therefore, these studies provide a function for C1D and suggest novel mechanisms for DNA-PK activation in vivo.

Bibliography

Yavuzer, U., Smith, G. C. M., Bliss, T., Werner, D., & Jackson, S. P. (1998). DNA end-independent activation of DNA-PK mediated via association with the DNA-binding proteinâC1D. Genes & Development, 12(14), 2188â2199.

Authors 5

Ugur Yavuzer (first)
Graeme C.M. Smith (additional)
Tonya Bliss (additional)
Dieter Werner (additional)
Stephen P. Jackson (additional)

References 52 Referenced 61

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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:18 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 13, 2024, 12:43 p.m.)
Issued	27 years, 1 month ago (July 15, 1998)
Published	27 years, 1 month ago (July 15, 1998)
Published Online	27 years, 1 month ago (July 15, 1998)
Published Print	27 years, 1 month ago (July 15, 1998)

Funders 0

None

BibTeX

@article{Yavuzer_1998, title={DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.14.2188}, DOI={10.1101/gad.12.14.2188}, number={14}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Yavuzer, Ugur and Smith, Graeme C.M. and Bliss, Tonya and Werner, Dieter and Jackson, Stephen P.}, year={1998}, month=jul, pages={2188–2199} }

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