DOI: 10.1101/gad.12.11.1652. Localization-dependent translation requires a functional interaction between the 5′ and 3′ ends of<i>oskar</i> mRNA

Localization-dependent translation requires a functional interaction between the 5′ and 3′ ends ofoskar mRNA

10.1101/gad.12.11.1652

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The precise restriction of proteins to specific domains within a cell plays an important role in early development and differentiation. An efficient way to localize and concentrate proteins is by localization of mRNA in a translationally repressed state, followed by activation of translation when the mRNA reaches its destination. A central issue is how localized mRNAs are derepressed. In this study we demonstrate that, when oskar mRNA reaches the posterior pole of the Drosophila oocyte, its translation is derepressed by an active process that requires a specific element in the 5′ region of the mRNA. We demonstrate that this novel type of element is a translational derepressor element, whose functional interaction with the previously identified repressor region in the oskar 3′ UTR is required for activation of oskar mRNA translation at the posterior pole. The derepressor element only functions at the posterior pole, suggesting that a locally restricted interaction betweentrans-acting factors and the derepressor element may be the link between mRNA localization and translational activation. We also show specific interaction of two proteins with the oskar mRNA 5′ region; one of these also recognizes the 3′ repressor element. We discuss the possible involvement of these factors as well as known genes in the process of localization-dependent translation.

Bibliography

Gunkel, N., Yano, T., Markussen, F.-H., Olsen, L. C., & Ephrussi, A. (1998). Localization-dependent translation requires a functional interaction between the 5â² and 3â² ends ofoskarâmRNA. Genes & Development, 12(11), 1652â1664.

Authors 5

Niki Gunkel (first)
Tamaki Yano (additional)
Finn-Hugo Markussen (additional)
Lisbeth C. Olsen (additional)
Anne Ephrussi (additional)

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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:52 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:15 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:18 p.m.)
Issued	27 years, 2 months ago (June 1, 1998)
Published	27 years, 2 months ago (June 1, 1998)
Published Online	27 years, 2 months ago (June 1, 1998)
Published Print	27 years, 2 months ago (June 1, 1998)

Funders 0

None

BibTeX

@article{Gunkel_1998, title={Localization-dependent translation requires a functional interaction between the 5′ and 3′ ends ofoskar mRNA}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.11.1652}, DOI={10.1101/gad.12.11.1652}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Gunkel, Niki and Yano, Tamaki and Markussen, Finn-Hugo and Olsen, Lisbeth C. and Ephrussi, Anne}, year={1998}, month=jun, pages={1652–1664} }

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