DOI: 10.1101/gad.12.10.1464. Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fission yeast

Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fission yeast

10.1101/gad.12.10.1464

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Control of gene expression by stress-activated protein kinase (SAPK) cascades is crucial for combating cytotoxic stress. Elements of these cascades have been investigated in detail, but regulation of stress signal transduction from the cytoplasm to the nucleus is poorly understood. Herein are reported subcellular localization studies of fission yeast Spc1, a homolog of human p38 and budding yeast Hog1p SAPKs. Stress induces transient nuclear localization of Spc1. Nuclear translocation of Spc1 is coupled with disassociation from its activator kinase Wis1. However, Spc1 does not concentrate in the nucleus ofΔwis1cells; therefore Wis1 does not tether Spc1 in the cytoplasm. Unphosphorylatable forms of Spc1 are dispersed in the cytoplasm and nucleus, even in cells that also produce wild-type Spc1. Thus, Spc1 must be phosphorylated by Wis1 to localize in the nucleus. Nuclear retention of Spc1 requires Atf1, a transcription factor that is the key nuclear substrate of Spc1. Nuclear localization of Atf1 requires Pcr1, a heterodimerization partner of Atf1. These studies show that phosphorylation and association with Atf1 are required for nuclear localization of Spc1.

Bibliography

Gaits, F., Degols, G., Shiozaki, K., & Russell, P. (1998). Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fissionâyeast. Genes & Development, 12(10), 1464â1473.

Authors 4

Frédérique Gaits (first)
Geneviève Degols (additional)
Kazuhiro Shiozaki (additional)
Paul Russell (additional)

References 39 Referenced 131

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{'key': '2021111418531626000_12.10.1464.6', 'first-page': '915', 'article-title': 'Nuclear localization and regulation of erk- and rsk-encoded protein kinases.', 'volume': '12', 'author': 'Chen', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Nuclear localization and regulation of erk- and rsk-encoded protein kinases. by Chen (1992)
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10.1128/MCB.16.6.2870 / Mol. Cell. Biol. / Activation and regulation of the Spc1 Mitogen-activated protein kinase in Schizosaccharomyces pombe. by Degols (1996)
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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:52 p.m.)
Deposited	1 year, 6 months ago (Feb. 22, 2024, 9:32 a.m.)
Indexed	5 days, 4 hours ago (Aug. 27, 2025, 11:41 a.m.)
Issued	27 years, 3 months ago (May 15, 1998)
Published	27 years, 3 months ago (May 15, 1998)
Published Online	27 years, 3 months ago (May 15, 1998)
Published Print	27 years, 3 months ago (May 15, 1998)

Funders 0

None

BibTeX

@article{Gaits_1998, title={Phosphorylation and association with the transcription factor Atf1 regulate localization of Spc1/Sty1 stress-activated kinase in fission yeast}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.10.1464}, DOI={10.1101/gad.12.10.1464}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Gaits, Frédérique and Degols, Geneviève and Shiozaki, Kazuhiro and Russell, Paul}, year={1998}, month=may, pages={1464–1473} }

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