DOI: 10.1101/gad.12.1.5. Nucleosome positioning by the winged helix transcription factor HNF3

Nucleosome positioning by the winged helix transcription factor HNF3

10.1101/gad.12.1.5

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Nucleosome positioning at genetic regulatory sequences is not well understood. The transcriptional enhancer of the mouse serum albumin gene is active in liver, where regulatory factors occupy their target sites on three nucleosome-like particles designated N1, N2, and N3. The winged helix transcription factor HNF3 binds to two sites near the center of the N1 particle. We created dinucleosome templates using the albumin enhancer sequence and found that site-specific binding of HNF3 protein resulted in nucleosome positioning in vitro similar to that seen in liver nuclei. Thus, binding of a transcription factor can position an underlying nucleosome core.

Bibliography

Shim, E. Y., Woodcock, C., & Zaret, K. S. (1998). Nucleosome positioning by the winged helix transcription factorâHNF3. Genes & Development, 12(1), 5â10.

Authors 3

Eun Yong Shim (first)
Christopher Woodcock (additional)
Kenneth S. Zaret (additional)

References 39 Referenced 98

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{'key': '2021111418502350000_12.1.5.2', 'first-page': '688', 'article-title': 'Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.', 'volume': '11', 'author': 'Archer', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter. by Archer (1991)
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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:12 p.m.)
Indexed	2 weeks ago (Aug. 23, 2025, 1:03 a.m.)
Issued	27 years, 8 months ago (Jan. 1, 1998)
Published	27 years, 8 months ago (Jan. 1, 1998)
Published Online	27 years, 8 months ago (Jan. 1, 1998)
Published Print	27 years, 8 months ago (Jan. 1, 1998)

Funders 0

None

BibTeX

@article{Shim_1998, title={Nucleosome positioning by the winged helix transcription factor HNF3}, volume={12}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.12.1.5}, DOI={10.1101/gad.12.1.5}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Shim, Eun Yong and Woodcock, Christopher and Zaret, Kenneth S.}, year={1998}, month=jan, pages={5–10} }

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