DOI: 10.1101/gad.1144403. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes

Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes

10.1101/gad.1144403

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Kinetochores are multiprotein complexes that assemble on centromeric DNA and attach chromosomes to spindle microtubules. Over the past six years, the number of proteins known to localize to the Saccharomyces cerevisiae kinetochore has increased from around 10 to over 60. However, relatively little is known about the protein-protein interactions that mediate kinetochore assembly or about the overall structure of microtubule-attachment sites. Here we used biophysical techniques, affinity purification, mass spectrometry, and in vivo assays to examine the state of association of 31 centromere-binding proteins, including six proteins newly identified as kinetochore subunits. We found that yeast kinetochores resemble transcriptional enhancers in being composed of at least 17 discrete subcomplexes that assemble on DNA to form a very large structure with a mass in excess of 5 MD. Critical to kinetochore assembly are proteins that bridge subunits in direct contact with DNA and subunits bound to microtubules. We show that two newly identified kinetochore complexes, COMA (Ctf19p-Okp1p-Mcm21p-Ame1p) and MIND (Mtw1p including Nnf1p-Nsl1p-Dsn1p) function as bridges. COMA, MIND, and the previously described Ndc80 complex constitute three independent and essential platforms onto which outer kinetochore proteins assemble. In addition, we propose that the three complexes have different functions with respect to force generation and MT attachment.

Bibliography

De Wulf, P., McAinsh, A. D., & Sorger, P. K. (2003). Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes & Development, 17(23), 2902â2921.

Authors 3

Peter De Wulf (first)
Andrew D. McAinsh (additional)
Peter K. Sorger (additional)

References 55 Referenced 247

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Dates

Type	When
Created	21 years, 9 months ago (Nov. 24, 2003, 8:25 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:36 p.m.)
Indexed	1 month, 3 weeks ago (July 12, 2025, 6:46 p.m.)
Issued	22 years, 8 months ago (Jan. 1, 2003)
Published	22 years, 8 months ago (Jan. 1, 2003)
Published Online	22 years, 8 months ago (Jan. 1, 2003)
Published Print	21 years, 9 months ago (Dec. 1, 2003)

Funders 0

None

BibTeX

@article{De_Wulf_2003, title={Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1144403}, DOI={10.1101/gad.1144403}, number={23}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={De Wulf, Peter and McAinsh, Andrew D. and Sorger, Peter K.}, year={2003}, month=jan, pages={2902–2921} }

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