Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis.
10.1101/gad.11.8.1061
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The zinc finger transcription factor GATA4 has been implicated in heart development based on its early expression in precardiogenic splanchnic mesoderm and its ability to activate the expression of a number of cardiac-specific genes. To determine the role of GATA4 in embryogenesis, we generated mice homozygous for a GATA4 null allele. Homozygous GATA4 null mice arrested in development between E7.0 and E9.5 because of severe developmental abnormalities. Mutant embryos most notably lacked a primitive heart tube and foregut and developed partially outside the yolk sac. In the mutants, the two bilaterally symmetric promyocardial primordia failed to migrate ventrally but instead remained lateral and generated two independent heart tubes that contained differentiated cardiomyocytes. We show that these deformities resulted from a general loss in lateral to ventral folding throughout the embryo. GATA4 is most highly expressed within the precardiogenic splanchnic mesoderm at the posterior lip of the anterior intestinal portal, corresponding to the region of the embryo that undergoes ventral fusion. We propose that GATA4 is required for the migration or folding morphogenesis of the precardiogenic splanchnic mesodermal cells at the level of the AIP.

Bibliography

Molkentin, J. D., Lin, Q., Duncan, S. A., & Olson, E. N. (1997). Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis. Genes & Development, 11(8), 1061–1072.

Authors 4
  1. J D Molkentin (first)
  2. Q Lin (additional)
  3. S A Duncan (additional)
  4. E N Olson (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 10:01 p.m.)
Indexed 3 weeks, 3 days ago (Aug. 6, 2025, 8:28 a.m.)
Issued 28 years, 4 months ago (April 15, 1997)
Published 28 years, 4 months ago (April 15, 1997)
Published Online 28 years, 4 months ago (April 15, 1997)
Published Print 28 years, 4 months ago (April 15, 1997)
Funders 0

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@article{Molkentin_1997, title={Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis.}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.8.1061}, DOI={10.1101/gad.11.8.1061}, number={8}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Molkentin, J D and Lin, Q and Duncan, S A and Olson, E N}, year={1997}, month=apr, pages={1061–1072} }