DOI: 10.1101/gad.11.7.876. The meiotic recombination hot spot created by the single-base substitution ade6-M26 results in remodeling of chromatin structure in fission yeast.

The meiotic recombination hot spot created by the single-base substitution ade6-M26 results in remodeling of chromatin structure in fission yeast.

10.1101/gad.11.7.876

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The G -->T transversion mutation, ade6-M26, creates the heptanucleotide sequence ATGACTG, which lies close to the 5' end of the open reading frame of the ade6 gene in Schizosaccharomyces pombe. The mutation generates a meiosis-specific recombination hot spot and a binding site for the Mts1/Mts2 protein. We examined the chromatin structure at the ade6 locus in the M26 strain and compared it to that of the wild-type and hot spot-negative control M375. Micrococcal nuclease (MNase) digestion and indirect end-labeling methods were applied. In the M26 strain, we detected a new MNase-hypersensitive site at the position of the M26 mutation and no longer observed the phasing of nucleosomes seen in the wild-type and the M375 strains. Quantitative comparison of MNase sensitivity of the chromatin in premeiotic and meiotic cultures revealed a small meiotic induction of MNase hypersensitivity in the ade6 promoter region of the wild-type and M375 strains. The meiotic induction of MNase hypersensitivity was enhanced significantly in the ade6 promoter region of the M26 strain and also occurred at the M26 mutation site. The formation of the MNase-sensitive region around the heptamer sequence was abolished by the introduction of single-nucleotide substitutions in the heptamer sequence, which also abolish hot spot activity and binding of Mts1/Mts2. These data suggest that Mts1/Mts2 binding to the heptamer sequence results in a chromatin structure suitable for the recruitment of a meiosis-specific recombination function or functions.

Bibliography

Mizuno, K., Emura, Y., Baur, M., Kohli, J., Ohta, K., & Shibata, T. (1997). The meiotic recombination hot spot created by the single-base substitution ade6-M26 results in remodeling of chromatin structure in fission yeast. Genes & Development, 11(7), 876â886.

Authors 6

K Mizuno (first)
Y Emura (additional)
M Baur (additional)
J Kohli (additional)
K Ohta (additional)
T Shibata (additional)

References 35 Referenced 76

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{'key': '2021111418435682000_11.7.876.9', 'first-page': '373', 'article-title': 'Timing of molecular events in meiosis in Saccharomyces cerevisiae. Stable heteroduplex DNA is formed late in meiotic prophase.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Timing of molecular events in meiosis in Saccharomyces cerevisiae. Stable heteroduplex DNA is formed late in meiotic prophase. (1993)
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{'key': '2021111418435682000_11.7.876.13', 'first-page': '2324', 'article-title': 'Physical detection of heterodu-plexes during meiotic recombination in the yeast Saccharomyces cerevisiae.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Physical detection of heterodu-plexes during meiotic recombination in the yeast Saccharomyces cerevisiae. (1993)
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10.1002/j.1460-2075.1992.tb05423.x / EMBO J. / Multiple sites for double-strand breaks in whole meiotic chromosomes of Saccharomyces cerevisiae. (1992)

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:01 p.m.)
Indexed	4 months, 4 weeks ago (April 5, 2025, 6:22 p.m.)
Issued	28 years, 5 months ago (April 1, 1997)
Published	28 years, 5 months ago (April 1, 1997)
Published Online	28 years, 5 months ago (April 1, 1997)
Published Print	28 years, 5 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Mizuno_1997, title={The meiotic recombination hot spot created by the single-base substitution ade6-M26 results in remodeling of chromatin structure in fission yeast.}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.7.876}, DOI={10.1101/gad.11.7.876}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Mizuno, K and Emura, Y and Baur, M and Kohli, J and Ohta, K and Shibata, T}, year={1997}, month=apr, pages={876–886} }

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