DOI: 10.1101/gad.11.5.654. Induction of c-myc transcription by the v-Abl tyrosine kinase requires Ras, Raf1, and cyclin-dependent kinases.

Induction of c-myc transcription by the v-Abl tyrosine kinase requires Ras, Raf1, and cyclin-dependent kinases.

10.1101/gad.11.5.654

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

v-Abl is an oncogenic form of the c-Abl nonreceptor tyrosine kinase. v-Abl induces transcription of c-myc, and c-Myc function is a necessary but not sufficient component of the v-Abl transformation program. Previously we showed that the E2F site in the c-myc promoter is a v-Abl response element and that v-Abl appears to induce c-myc by initiating a phosphorylation cascade that ultimately activates E2F-binding proteins. In this work we have investigated the signaling pathway between the v-Abl tyrosine kinase and activated E2F proteins. We show that the Ras GTPase and Raf1 serine/threonine kinase are required in this pathway. However, in contrast to other aspects of v-Abl signaling, induction of c-myc transcription is independent of the Rac GTPase. Our results also establish a requirement for activated cyclin-dependent kinases (cdks), as v-Abl-dependent induction of c-myc transcription is blocked by cdk inhibitor p21 and induction of c-myc is accompanied by activation of cdk2 and cdk4. Finally, we show that v-Abl-dependent induction of c-myc is accompanied by hyperphosphorylation of pRb, p107, and p130. On the basis of these data, we propose a model for the signaling path from v-Abl to c-myc.

Bibliography

Zou, X., Rudchenko, S., Wong, K., & Calame, K. (1997). Induction of c-myc transcription by the v-Abl tyrosine kinase requires Ras, Raf1, and cyclin-dependent kinases. Genes & Development, 11(5), 654â662.

Authors 4

X Zou (first)
S Rudchenko (additional)
K Wong (additional)
K Calame (additional)

References 48 Referenced 60

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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:58 p.m.)
Indexed	1 year, 4 months ago (April 17, 2024, 6:30 a.m.)
Issued	28 years, 5 months ago (March 1, 1997)
Published	28 years, 5 months ago (March 1, 1997)
Published Online	28 years, 5 months ago (March 1, 1997)
Published Print	28 years, 5 months ago (March 1, 1997)

Funders 0

None

BibTeX

@article{Zou_1997, title={Induction of c-myc transcription by the v-Abl tyrosine kinase requires Ras, Raf1, and cyclin-dependent kinases.}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.5.654}, DOI={10.1101/gad.11.5.654}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Zou, X and Rudchenko, S and Wong, K and Calame, K}, year={1997}, month=mar, pages={654–662} }

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