DOI: 10.1101/gad.11.24.3432. Calcineurin acts through the<i>CRZ1/TCN1</i>-encoded transcription factor to regulate gene expression in yeast

Calcineurin acts through theCRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast

10.1101/gad.11.24.3432

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Calcineurin is a conserved Ca2+/calmodulin-dependent protein phosphatase that plays a critical role in Ca2+ signaling. We describe new components of a calcineurin-mediated response in yeast, the Ca2+-induced transcriptional activation ofFKS2, which encodes a β-1,3 glucan synthase. A 24-bp region of the FKS2 promoter was defined as sufficient to confer calcineurin-dependent transcriptional induction on a minimal promoter in response to Ca2+ and was named CDRE (forcalcineurin-dependentresponse element). The product ofCRZ1 (YNL027w) was identified as an activator of CDRE-driven transcription. Crz1p contains zinc finger motifs and binds specifically to the CDRE. Genetic analysis revealed that crz1Δ mutant cells exhibit several phenotypes similar to those of calcineurin mutants and that overexpression of CRZ1 in calcineurin mutants suppressed these phenotypes. These results suggest that Crz1p functions downstream of calcineurin to effect multiple calcineurin-dependent responses. Moreover, the calcineurin-dependent transcriptional induction of FKS2 in response to Ca2+, α-factor, and Na+ was found to require CRZ1. In addition, we found that the calcineurin-dependent transcriptional regulation ofPMR2 and PMC1 required CRZ1. However, transcription of PMR2 and PMC1 was activated by only a subset of the treatments that activated FKS2 transcription. Thus, in response to multiple signals, calcineurin acts through the Crz1p transcription factor to differentially regulate the expression of several target genes in yeast.

Bibliography

Stathopoulos, A. M., & Cyert, M. S. (1997). Calcineurin acts through theCRZ1/TCN1-encoded transcription factor to regulate gene expression inâyeast. Genes & Development, 11(24), 3432â3444.

Authors 2

Angelike M. Stathopoulos (first)
Martha S. Cyert (additional)

References 55 Referenced 404

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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:12 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:25 a.m.)
Issued	27 years, 8 months ago (Dec. 15, 1997)
Published	27 years, 8 months ago (Dec. 15, 1997)
Published Online	27 years, 8 months ago (Dec. 15, 1997)
Published Print	27 years, 8 months ago (Dec. 15, 1997)

Funders 0

None

BibTeX

@article{Stathopoulos_1997, title={Calcineurin acts through theCRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.24.3432}, DOI={10.1101/gad.11.24.3432}, number={24}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Stathopoulos, Angelike M. and Cyert, Martha S.}, year={1997}, month=dec, pages={3432–3444} }

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