DOI: 10.1101/gad.11.23.3116. Disruption of downstream chromatin directed by a transcriptional activator

Disruption of downstream chromatin directed by a transcriptional activator

10.1101/gad.11.23.3116

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Promoter–proximal pausing during transcriptional elongation is an important way of regulating many diverse loci, including the humanhsp70 gene. Pausing of RNA polymerase can be enhanced by chromatin structure. We demonstrate that activation of hsp70leads to disruption of transcribed chromatin in front of RNA polymerase. In vivo, disruption of chromatin in the first 400 bp of the transcribed region of hsp70 following heat shock is resistant to the transcriptional inhibitor α-amanitin. Disruption of chromatin farther downstream also occurs following activation but is sensitive to α-amanitin, suggesting that polymerase movement is needed to disrupt distal portions of the hsp70 gene. In vitro, disruption of transcribed chromatin is dependent on the presence of the human heat shock factor 1 (HSF1) activation domains. These experiments demonstrate that HSF1 can direct disruption of chromatin in transcribed regions. We suggest that this is one of the mechanisms used by HSF1 to facilitate transcriptional elongation.

Bibliography

Brown, S. A., & Kingston, R. E. (1997). Disruption of downstream chromatin directed by a transcriptionalâactivator. Genes & Development, 11(23), 3116â3121.

Authors 2

Steven A. Brown (first)
Robert E. Kingston (additional)

References 28 Referenced 53

Ausubel F.M. Brent R. Kingston R.E. Moore D.D. Seidman J.G. Smith J.A. Struhl K. (1995) Current protocols in molecular biology. (Wiley and Sons, New York, NY).
10.1101/gad.7.5.857
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10.1101/gad.10.12.1479
10.1016/0092-8674(92)90262-B
10.1074/jbc.271.38.23352
10.1101/gad.11.4.436
10.1101/gad.6.11.2190
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10.1128/MCB.16.4.1614 / Mol. Cell. Biol. / A three-step pathway of transcription initiation leading to promoter clearance at an activated RNA polymerase II promoter. by Jiang (1996)
10.1101/gad.9.5.559
10.1128/MCB.16.10.5433 / Mol. Cell. Biol. / Analyses of promoter-proximal pausing by RNA polymerase II on the hsp70 heat shock gene promoter in a Drosophila nuclear extract. by Li (1996)
10.1016/0092-8674(93)90286-Y
10.1002/j.1460-2075.1989.tb08362.x / EMBO J. / Nucleosomes inhibit both transcriptional initiation and elongation by RNA polymerase III in vitro. by Morse (1989)
10.1126/science.2814500
10.1101/gad.9.11.1366
{'key': '2021111418494641000_11.23.3116.18', 'first-page': '3456', 'article-title': 'Rapid changes in Drosophila transcription after instantaneous heat shock.', 'volume': '13', 'author': 'Oâ\x80\x99Brien', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Rapid changes in Drosophila transcription after instantaneous heat shock. by OâBrien (1993)
10.1101/gad.8.7.830
10.1073/pnas.90.17.7923
10.1016/S0092-8674(88)91087-2
10.1074/jbc.271.35.21549
{'key': '2021111418494641000_11.23.3116.23', 'first-page': '777', 'article-title': 'Transcription elongation and eukaryotic gene regulation.', 'volume': '5', 'author': 'Spencer', 'year': '1990', 'journal-title': 'Oncogene'} / Oncogene / Transcription elongation and eukaryotic gene regulation. by Spencer (1990)
10.1016/0092-8674(94)90343-3
10.1016/0092-8674(95)90216-3
10.1016/S0092-8674(00)80978-2
{'key': '2021111418494641000_11.23.3116.27', 'first-page': '324', 'article-title': 'Rapid, small-scale RNA isolation from tissue culture cells.', 'volume': '11', 'author': 'Xie', 'year': '1991', 'journal-title': 'BioTechniques'} / BioTechniques / Rapid, small-scale RNA isolation from tissue culture cells. by Xie (1991)
10.1016/0092-8674(94)90058-2

Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:11 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 5:16 p.m.)
Issued	27 years, 8 months ago (Dec. 1, 1997)
Published	27 years, 8 months ago (Dec. 1, 1997)
Published Online	27 years, 8 months ago (Dec. 1, 1997)
Published Print	27 years, 8 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Brown_1997, title={Disruption of downstream chromatin directed by a transcriptional activator}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.23.3116}, DOI={10.1101/gad.11.23.3116}, number={23}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Brown, Steven A. and Kingston, Robert E.}, year={1997}, month=dec, pages={3116–3121} }

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