DOI: 10.1101/gad.11.19.2569. A novel histidine kinase inhibitor regulating development in<i>Bacillus subtilis</i>

A novel histidine kinase inhibitor regulating development inBacillus subtilis

10.1101/gad.11.19.2569

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Kinase A is the sensor histidine kinase responsible for processing postexponential phase information and providing phosphate input to the phosphorelay that activates developmental transcription via phosphorylated Spo0A. A protein inhibitor, KipI, of kinase A was discovered encoded in an operon of genes of unknown function but regulated by the availability of fixed nitrogen. KipI is a potent inhibitor of the autophosphorylation reaction of kinase A but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase A affecting the ATP/ADP reactions and not the phosphotransferase functions of this domain. The inhibitory activity of KipI is counteracted by the product of another gene in the operon, KipA. This protein may bind to KipI, preventing its function as an inhibitor of kinase A. KipI may be the first representative of a new class of signal transduction inhibitors that function by direct interaction with the catalytic domain of histidine kinases to counteract signals influencing the “sensor” domain of such kinases. This inhibitor represents yet another way by which the phosphorelay signal transduction system is affected by negative regulators under the control of metabolic, environmental, or cell cycle influences antithetical to the initiation of developmental transcription.

Bibliography

Wang, L., Grau, R., Perego, M., & Hoch, J. A. (1997). A novel histidine kinase inhibitor regulating development inBacillusâsubtilis. Genes & Development, 11(19), 2569â2579.

Authors 4

Ling Wang (first)
Roberto Grau (additional)
Marta Perego (additional)
James A. Hoch (additional)

References 32 Referenced 122

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10.1007/BF00391754
Magasanik B. (1992) Regulation of nitrogen utilization. in The molecular and cellular biology of the yeast Saccharomyces cerevisiae: Metabolism and gene expression, ed Stratherm (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY), pp 283–317.
Miller J.H. (1972) Experiments in molecular genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), pp 352–355.
10.1073/pnas.83.16.5909
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Perego M. (1993) Bacillus subtilis and other Gram-positive bacteria: Biochemistry, physiology, and molecular genetics. in Integrational vectors for genetic manipulation in Bacillus subtilis, eds Sonenshein A.L. Hoch J.A. Losick R. (American Society for Microbiology, Washington, D.C.) pp 615–624. (10.1128/9781555818388.ch42)
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10.1128/jb.171.11.6187-6196.1989 / J. Bacteriol. / Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis. by Perego (1989)
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10.1111/j.1365-2958.1996.tb02460.x
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{'key': '2021111418473748000_11.19.2569.25', 'first-page': '337', 'article-title': 'Molecular characterization of the lam locus and sequences involved in regulation by the AmdR protein of Aspergillus nidulans.', 'volume': '12', 'author': 'Richardson', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Molecular characterization of the lam locus and sequences involved in regulation by the AmdR protein of Aspergillus nidulans. by Richardson (1992)
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{'key': '2021111418473748000_11.19.2569.32', 'first-page': '2926', 'article-title': 'A novel phosphorylation activity of the Bacillus subtilis sporulation protein Spo0F that employs phosphoramidate substrates.', 'volume': '35', 'author': 'Zapf', 'year': '1996', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / A novel phosphorylation activity of the Bacillus subtilis sporulation protein Spo0F that employs phosphoramidate substrates. by Zapf (1996)

Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:08 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 19, 2025, 7:05 a.m.)
Issued	27 years, 11 months ago (Oct. 1, 1997)
Published	27 years, 11 months ago (Oct. 1, 1997)
Published Online	27 years, 11 months ago (Oct. 1, 1997)
Published Print	27 years, 11 months ago (Oct. 1, 1997)

Funders 0

None

BibTeX

@article{Wang_1997, title={A novel histidine kinase inhibitor regulating development inBacillus subtilis}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.19.2569}, DOI={10.1101/gad.11.19.2569}, number={19}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Wang, Ling and Grau, Roberto and Perego, Marta and Hoch, James A.}, year={1997}, month=oct, pages={2569–2579} }

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