DOI: 10.1101/gad.11.19.2482. A multifunctional DNA-binding protein that promotes the formation of serum response factor/homeodomain complexes: identity to TFII-I

A multifunctional DNA-binding protein that promotes the formation of serum response factor/homeodomain complexes: identity to TFII-I

10.1101/gad.11.19.2482

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The human homeodomain protein Phox1 interacts functionally with serum response factor (SRF) to impart serum responsive transcriptional activity to SRF-binding sites in a HeLa cell cotransfection assay. However, stable ternary complexes composed of SRF, Phox1, and DNA, which presumably mediate the transcriptional effects of Phox1 in vivo, have not been observed in vitro. Here, we report the identification, purification, and molecular cloning of a human protein that promotes the formation of stable higher-order complexes of SRF and Phox1. We show that this protein, termed SPIN, interacts with SRF and Phox1 in vitro and in vivo. Furthermore, SPIN binds specifically to multiple sequences in the c-fos promoter and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the c-fos serum response element (SRE). SPIN is identical to the initiator-binding protein TFII-I. Consistent with this hypothesis, SPIN exhibits modest affinity for a characterized initiator sequence in vitro. We propose that this multifunctional protein coordinates the formation of an active promoter complex at the c-fos gene, including the linkage of specific signal responsive activator complexes to the general transcription machinery.

Bibliography

Grueneberg, D. A., Henry, R. W., Brauer, A., Novina, C. D., Cheriyath, V., Roy, A. L., & Gilman, M. (1997). A multifunctional DNA-binding protein that promotes the formation of serum response factor/homeodomain complexes: identity toâTFII-I. Genes & Development, 11(19), 2482â2493.

Authors 7

Dorre A. Grueneberg (first)
R. William Henry (additional)
Andrew Brauer (additional)
Carl D. Novina (additional)
Venugopalan Cheriyath (additional)
Ananda L. Roy (additional)
Michael Gilman (additional)

References 41 Referenced 113

{'key': '2021111418473748000_11.19.2482.1', 'first-page': '2432', 'article-title': 'Expression cloning of a novel zinc-finger protein that binds to the c-fos serum response element.', 'volume': '12', 'author': 'Attar', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Expression cloning of a novel zinc-finger protein that binds to the c-fos serum response element. by Attar (1992)
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Dates

Type	When
Created	17 years, 6 months ago (Feb. 20, 2008, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:08 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 9:05 a.m.)
Issued	27 years, 10 months ago (Oct. 1, 1997)
Published	27 years, 10 months ago (Oct. 1, 1997)
Published Online	27 years, 10 months ago (Oct. 1, 1997)
Published Print	27 years, 10 months ago (Oct. 1, 1997)

Funders 0

None

BibTeX

@article{Grueneberg_1997, title={A multifunctional DNA-binding protein that promotes the formation of serum response factor/homeodomain complexes: identity to TFII-I}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.19.2482}, DOI={10.1101/gad.11.19.2482}, number={19}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Grueneberg, Dorre A. and Henry, R. William and Brauer, Andrew and Novina, Carl D. and Cheriyath, Venugopalan and Roy, Ananda L. and Gilman, Michael}, year={1997}, month=oct, pages={2482–2493} }

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          1997,
          10,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.11.19.2482"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.11.19.2482",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1997,
        10,
        1
      ]
    ]
  }
}