The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins.
10.1101/gad.11.13.1703
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Cleavage and polyadenylation specificity factor (CPSF), a key component of the mammalian RNA 3'-end processing machinery, consists of four subunits of 160, 100, 73, and 30 kD. Here we report the isolation and characterization of a cDNA encoding the 30-kD polypeptide. Antibodies raised against this protein inhibit cleavage and polyadenylation and coimmunoprecipitate the other CPSF subunits. The protein sequence contains five C3H-zinc-finger repeats and a putative RNA-binding zinc knuckle motif at the carboxyl terminus. Consistent with this observation, the in vitro translated 30-kD protein binds RNA polymers with a distinct preference for poly(U). In addition, an essential S. cerevisiae gene, YTH1, was cloned which is 40% identical to CPSF 30K at the protein level. Extracts prepared from a conditional yth1 mutant have normal cleavage activity, but fail to polyadenylate the upstream cleavage product. Efficient polyadenylation activity can be restored by the addition of purified polyadenylation factor I (PF I). We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit.

Bibliography

Barabino, S. M., Hübner, W., Jenny, A., Minvielle-Sebastia, L., & Keller, W. (1997). The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes & Development, 11(13), 1703–1716.

Authors 5
  1. S M Barabino (first)
  2. W Hübner (additional)
  3. A Jenny (additional)
  4. L Minvielle-Sebastia (additional)
  5. W Keller (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 10:03 p.m.)
Indexed 2 weeks, 6 days ago (Aug. 5, 2025, 8:22 a.m.)
Issued 28 years, 1 month ago (July 1, 1997)
Published 28 years, 1 month ago (July 1, 1997)
Published Online 28 years, 1 month ago (July 1, 1997)
Published Print 28 years, 1 month ago (July 1, 1997)
Funders 0

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@article{Barabino_1997, title={The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins.}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.13.1703}, DOI={10.1101/gad.11.13.1703}, number={13}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Barabino, S M and Hübner, W and Jenny, A and Minvielle-Sebastia, L and Keller, W}, year={1997}, month=jul, pages={1703–1716} }