DOI: 10.1101/gad.11.1.129. The alternative splicing factor PSI regulates P-element third intron splicing in vivo.

The alternative splicing factor PSI regulates P-element third intron splicing in vivo.

10.1101/gad.11.1.129

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Splicing of the Drosophila P-element third intron (IVS3) is inhibited in somatic cells, restricting transposase expression to the germ line. Somatic inhibition of IVS3 splicing involves the assembly of a multiprotein complex on a regulatory sequence in the IVS3 5' exon. The P-element somatic inhibitor protein (PSI) is a component of this ribonucleoprotein complex and is required for inhibition of IVS3 splicing in vitro. The soma-specific expression pattern of PSI suggests that its low abundance in the germ line allows IVS3 splicing. We demonstrate that ectopic expression of PSI in the female germ line is sufficient to repress splicing of an IVS3 reporter transgene. We also show that IVS3 splicing is activated in somatic embryonic cells in the presence of an antisense PSI ribozyme. These results support the model that PSI is a tissue-specific regulator of IVS3 splicing in vivo.

Bibliography

Adams, M. D., Tarng, R. S., & Rio, D. C. (1997). The alternative splicing factor PSI regulates P-element third intron splicing in vivo. Genes & Development, 11(1), 129â138.

Authors 3

M D Adams (first)
R S Tarng (additional)
D C Rio (additional)

References 34 Referenced 50

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{'key': '2021111418420476000_11.1.129.5', 'first-page': '1538', 'article-title': 'Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron. (1991)
{'key': '2021111418420476000_11.1.129.6', 'first-page': '3773', 'article-title': 'Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis. (1993)
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10.1038/365448a0

Dates

Type	When
Created	18 years, 3 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:55 p.m.)
Indexed	1 week, 1 day ago (Aug. 29, 2025, 6:28 a.m.)
Issued	28 years, 8 months ago (Jan. 1, 1997)
Published	28 years, 8 months ago (Jan. 1, 1997)
Published Online	28 years, 8 months ago (Jan. 1, 1997)
Published Print	28 years, 8 months ago (Jan. 1, 1997)

Funders 0

None

BibTeX

@article{Adams_1997, title={The alternative splicing factor PSI regulates P-element third intron splicing in vivo.}, volume={11}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.11.1.129}, DOI={10.1101/gad.11.1.129}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Adams, M D and Tarng, R S and Rio, D C}, year={1997}, month=jan, pages={129–138} }

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