DOI: 10.1101/gad.1060403. Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing

Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing

10.1101/gad.1060403

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3′-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3′-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an α-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3′-end formation.

Bibliography

Szymczyna, B. R., Bowman, J., McCracken, S., Pineda-Lucena, A., Lu, Y., Cox, B., Lambermon, M., Graveley, B. R., Arrowsmith, C. H., & Blencowe, B. J. (2003). Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing. Genes & Development, 17(4), 461â475.

Authors 10

Blair R. Szymczyna (first)
John Bowman (additional)
Susan McCracken (additional)
Antonio Pineda-Lucena (additional)
Ying Lu (additional)
Brian Cox (additional)
Mark Lambermon (additional)
Brenton R. Graveley (additional)
Cheryl H. Arrowsmith (additional)
Benjamin J. Blencowe (additional)

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Dates

Type	When
Created	22 years, 6 months ago (Feb. 24, 2003, 6:03 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:13 p.m.)
Indexed	3 weeks, 5 days ago (July 30, 2025, 10:07 a.m.)
Issued	22 years, 6 months ago (Feb. 15, 2003)
Published	22 years, 6 months ago (Feb. 15, 2003)
Published Online	22 years, 6 months ago (Feb. 15, 2003)
Published Print	22 years, 6 months ago (Feb. 15, 2003)

Funders 0

None

BibTeX

@article{Szymczyna_2003, title={Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1060403}, DOI={10.1101/gad.1060403}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Szymczyna, Blair R. and Bowman, John and McCracken, Susan and Pineda-Lucena, Antonio and Lu, Ying and Cox, Brian and Lambermon, Mark and Graveley, Brenton R. and Arrowsmith, Cheryl H. and Blencowe, Benjamin J.}, year={2003}, month=feb, pages={461–475} }

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