DOI: 10.1101/gad.1056603. Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin

Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin

10.1101/gad.1056603

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Drosophila Enhancer of Polycomb, E(Pc), is a suppressor of position-effect variegation and an enhancer of both Polycomb and trithorax mutations. A homologous yeast protein, Epl1, is a subunit of the NuA4 histone acetyltransferase complex. Epl1 depletion causes cells to accumulate in G2/M and global loss of acetylated histones H4 and H2A. In relation to theDrosophilaprotein, mutation of Epl1 suppresses gene silencing by telomere position effect. Epl1 protein is found in the NuA4 complex and a novel highly active smaller complex named Piccolo NuA4 (picNuA4). The picNuA4 complex contains Esa1, Epl1, and Yng2 as subunits and strongly prefers chromatin over free histones as substrate. Epl1 conserved N-terminal domain bridges Esa1 and Yng2 together, stimulating Esa1 catalytic activity and enabling acetylation of chromatin substrates. A recombinant picNuA4 complex shows characteristics similar to the native complex, including strong chromatin preference. Cells expressing only the N-terminal half of Epl1 lack NuA4 HAT activity, but possess picNuA4 complex and activity. These results indicate that the essential aspect of Esa1 and Epl1 resides in picNuA4 function. We propose that picNuA4 represents a nontargeted histone H4/H2A acetyltransferase activity responsible for global acetylation, whereas the NuA4 complex is recruited to specific genomic loci to perturb locally the dynamic acetylation/deacetylation equilibrium.

Bibliography

Boudreault, A. A., Cronier, D., Selleck, W., Lacoste, N., Utley, R. T., Allard, S., Savard, J., Lane, W. S., Tan, S., & CÃ´tÃ©, J. (2003). Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin. Genes & Development, 17(11), 1415â1428.

Authors 10

Alexandre A. Boudreault (first)
Dominique Cronier (additional)
William Selleck (additional)
Nicolas Lacoste (additional)
Rhea T. Utley (additional)
Stéphane Allard (additional)
Julie Savard (additional)
William S. Lane (additional)
Song Tan (additional)
Jacques Côté (additional)

References 38 Referenced 182

Adams A ., Gottschling, D.E., Kaiser, C.A., and Stearns, T. 1997. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
10.1093/emboj/18.18.5108
10.1074/jbc.M110849200
10.1038/nature01035
10.1126/science.1060214
10.1074/jbc.M102531200
10.1128/MCB.19.4.2515 / Mol. Cell. Biol. (1999)
10.1073/pnas.95.9.4947
10.1074/jbc.M008159200
10.1016/S1097-2765(00)80258-0
10.1091/mbc.11.12.4241
10.1093/genetics/156.2.645 / Genetics (2000)
10.1016/0092-8674(90)90141-Z
10.1101/gad.11.13.1640
10.1128/MCB.22.14.5047-5053.2002
10.1126/science.1063127
10.1101/gad.967302
10.1128/MCB.20.11.3807-3816.2000
10.1074/jbc.M102176200
10.1101/gad.9.14.1716
10.1128/MCB.21.22.7629-7640.2001
10.1128/MCB.19.3.2061 / Mol. Cell. Biol. (1999)
10.1101/gad.207401
10.1006/meth.2001.1183
10.1016/S1097-2765(00)00128-3
10.1074/jbc.272.9.5571
10.1074/jbc.M003783200
10.1074/jbc.M006585200
10.1093/genetics/148.1.211 / Genetics (1998)
10.1242/dev.125.20.4055 / Development (1998)
10.1016/S1097-2765(01)00301-X
10.1006/prep.2000.1363
{'key': '2021111519023242000_17.11.1415.33', 'first-page': '203', 'volume': '274', 'year': '2002', 'journal-title': 'Curr. Topics Microbiol. Immunol.'} / Curr. Topics Microbiol. Immunol. (2002)
10.1038/28886
10.1093/emboj/21.5.1101
10.1093/emboj/19.11.2629
10.1038/35044127
10.1139/o02-080

Dates

Type	When
Created	22 years, 2 months ago (June 3, 2003, 9:54 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:08 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 3:08 p.m.)
Issued	22 years, 2 months ago (June 1, 2003)
Published	22 years, 2 months ago (June 1, 2003)
Published Online	22 years, 2 months ago (June 1, 2003)
Published Print	22 years, 2 months ago (June 1, 2003)

Funders 0

None

BibTeX

@article{Boudreault_2003, title={Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1056603}, DOI={10.1101/gad.1056603}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Boudreault, Alexandre A. and Cronier, Dominique and Selleck, William and Lacoste, Nicolas and Utley, Rhea T. and Allard, Stéphane and Savard, Julie and Lane, William S. and Tan, Song and Côté, Jacques}, year={2003}, month=jun, pages={1415–1428} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T19:08:02Z",
    "timestamp": 1751483282468
  },
  "reference-count": 38,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "11",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        6,
        1
      ]
    ]
  },
  "abstract": "<jats:p><jats:italic>Drosophila Enhancer of Polycomb, E(Pc)</jats:italic>, is a suppressor of position-effect variegation and an enhancer of both Polycomb and trithorax mutations. A homologous yeast protein, Epl1, is a subunit of the NuA4 histone acetyltransferase complex. Epl1 depletion causes cells to accumulate in G2/M and global loss of acetylated histones H4 and H2A. In relation to the<jats:italic>Drosophila</jats:italic>protein, mutation of Epl1 suppresses gene silencing by telomere position effect. Epl1 protein is found in the NuA4 complex and a novel highly active smaller complex named Piccolo NuA4 (picNuA4). The picNuA4 complex contains Esa1, Epl1, and Yng2 as subunits and strongly prefers chromatin over free histones as substrate. Epl1 conserved N-terminal domain bridges Esa1 and Yng2 together, stimulating Esa1 catalytic activity and enabling acetylation of chromatin substrates. A recombinant picNuA4 complex shows characteristics similar to the native complex, including strong chromatin preference. Cells expressing only the N-terminal half of Epl1 lack NuA4 HAT activity, but possess picNuA4 complex and activity. These results indicate that the essential aspect of Esa1 and Epl1 resides in picNuA4 function. We propose that picNuA4 represents a nontargeted histone H4/H2A acetyltransferase activity responsible for global acetylation, whereas the NuA4 complex is recruited to specific genomic loci to perturb locally the dynamic acetylation/deacetylation equilibrium.</jats:p>",
  "DOI": "10.1101/gad.1056603",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        6,
        4
      ]
    ],
    "date-time": "2003-06-04T01:54:18Z",
    "timestamp": 1054691658000
  },
  "page": "1415-1428",
  "source": "Crossref",
  "is-referenced-by-count": 182,
  "title": "Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin",
  "prefix": "10.1101",
  "volume": "17",
  "author": [
    {
      "given": "Alexandre A.",
      "family": "Boudreault",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Dominique",
      "family": "Cronier",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "William",
      "family": "Selleck",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Nicolas",
      "family": "Lacoste",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Rhea T.",
      "family": "Utley",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "St\u00e9phane",
      "family": "Allard",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Julie",
      "family": "Savard",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "William S.",
      "family": "Lane",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Song",
      "family": "Tan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jacques",
      "family": "C\u00f4t\u00e9",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        2003,
        6,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111519023242000_17.11.1415.1",
      "unstructured": "Adams A ., Gottschling, D.E., Kaiser, C.A., and Stearns, T. 1997. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."
    },
    {
      "key": "2021111519023242000_17.11.1415.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/18.18.5108"
    },
    {
      "key": "2021111519023242000_17.11.1415.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M110849200"
    },
    {
      "key": "2021111519023242000_17.11.1415.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature01035"
    },
    {
      "key": "2021111519023242000_17.11.1415.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1060214"
    },
    {
      "key": "2021111519023242000_17.11.1415.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M102531200"
    },
    {
      "key": "2021111519023242000_17.11.1415.7",
      "doi-asserted-by": "crossref",
      "first-page": "2515",
      "DOI": "10.1128/MCB.19.4.2515",
      "volume": "19",
      "year": "1999",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111519023242000_17.11.1415.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.9.4947"
    },
    {
      "key": "2021111519023242000_17.11.1415.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M008159200"
    },
    {
      "key": "2021111519023242000_17.11.1415.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80258-0"
    },
    {
      "key": "2021111519023242000_17.11.1415.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.11.12.4241"
    },
    {
      "key": "2021111519023242000_17.11.1415.12",
      "doi-asserted-by": "crossref",
      "first-page": "645",
      "DOI": "10.1093/genetics/156.2.645",
      "volume": "156",
      "year": "2000",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111519023242000_17.11.1415.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90141-Z"
    },
    {
      "key": "2021111519023242000_17.11.1415.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.11.13.1640"
    },
    {
      "key": "2021111519023242000_17.11.1415.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.22.14.5047-5053.2002"
    },
    {
      "key": "2021111519023242000_17.11.1415.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1063127"
    },
    {
      "key": "2021111519023242000_17.11.1415.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.967302"
    },
    {
      "key": "2021111519023242000_17.11.1415.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.20.11.3807-3816.2000"
    },
    {
      "key": "2021111519023242000_17.11.1415.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M102176200"
    },
    {
      "key": "2021111519023242000_17.11.1415.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.14.1716"
    },
    {
      "key": "2021111519023242000_17.11.1415.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.21.22.7629-7640.2001"
    },
    {
      "key": "2021111519023242000_17.11.1415.22",
      "doi-asserted-by": "crossref",
      "first-page": "2061",
      "DOI": "10.1128/MCB.19.3.2061",
      "volume": "19",
      "year": "1999",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111519023242000_17.11.1415.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.207401"
    },
    {
      "key": "2021111519023242000_17.11.1415.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/meth.2001.1183"
    },
    {
      "key": "2021111519023242000_17.11.1415.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)00128-3"
    },
    {
      "key": "2021111519023242000_17.11.1415.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.9.5571"
    },
    {
      "key": "2021111519023242000_17.11.1415.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M003783200"
    },
    {
      "key": "2021111519023242000_17.11.1415.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M006585200"
    },
    {
      "key": "2021111519023242000_17.11.1415.29",
      "doi-asserted-by": "crossref",
      "first-page": "211",
      "DOI": "10.1093/genetics/148.1.211",
      "volume": "148",
      "year": "1998",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111519023242000_17.11.1415.30",
      "doi-asserted-by": "crossref",
      "first-page": "4055",
      "DOI": "10.1242/dev.125.20.4055",
      "volume": "125",
      "year": "1998",
      "journal-title": "Development"
    },
    {
      "key": "2021111519023242000_17.11.1415.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00301-X"
    },
    {
      "key": "2021111519023242000_17.11.1415.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/prep.2000.1363"
    },
    {
      "key": "2021111519023242000_17.11.1415.33",
      "first-page": "203",
      "volume": "274",
      "year": "2002",
      "journal-title": "Curr. Topics Microbiol. Immunol."
    },
    {
      "key": "2021111519023242000_17.11.1415.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/28886"
    },
    {
      "key": "2021111519023242000_17.11.1415.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/21.5.1101"
    },
    {
      "key": "2021111519023242000_17.11.1415.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.11.2629"
    },
    {
      "key": "2021111519023242000_17.11.1415.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35044127"
    },
    {
      "key": "2021111519023242000_17.11.1415.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1139/o02-080"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.1056603",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        16
      ]
    ],
    "date-time": "2021-11-16T03:08:06Z",
    "timestamp": 1637032086000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.1056603"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        6,
        1
      ]
    ]
  },
  "references-count": 38,
  "journal-issue": {
    "issue": "11",
    "published-print": {
      "date-parts": [
        [
          2003,
          6,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.1056603"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.1056603",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        2003,
        6,
        1
      ]
    ]
  }
}