DOI: 10.1101/gad.1048103. A biochemical framework for RNA silencing in plants

A biochemical framework for RNA silencing in plants

10.1101/gad.1048103

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

RNA silencing phenomena were first discovered in plants, yet only the RNA interference pathway in animals has been subject to biochemical analysis. Here, we extend biochemical analysis to plant RNA silencing. We find that standard wheat germ extract contains Dicer-like enzymes that convert double-stranded RNA (dsRNA) into two classes of small interfering RNAs, as well as an RNA-dependent RNA polymerase activity that can convert exogenous single-stranded RNA into dsRNA. In this plant embryo extract, an endogenous microRNA (miRNA) that lacks perfect complementarity to its RNA targets nonetheless acts as a small interfering RNA. The miRNA guides an endonuclease to cleave efficiently wild-typeArabidopsis PHAVOLUTAmRNA, but not a dominant mutant previously shown to perturb leaf development. This finding supports the view that plant miRNAs direct RNAi and that miRNA-specified mRNA destruction is important for proper plant development. Thus, endonuclease complexes guided by small RNAs are a common feature of RNA silencing in both animals and plants.

Bibliography

Tang, G., Reinhart, B. J., Bartel, D. P., & Zamore, P. D. (2003). A biochemical framework for RNA silencing in plants. Genes & Development, 17(1), 49â63.

Authors 4

Guiliang Tang (first)
Brenda J. Reinhart (additional)
David P. Bartel (additional)
Phillip D. Zamore (additional)

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Dates

Type	When
Created	22 years, 7 months ago (Jan. 3, 2003, 1:03 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:05 p.m.)
Indexed	4 days, 10 hours ago (Aug. 27, 2025, 12:36 p.m.)
Issued	22 years, 7 months ago (Jan. 1, 2003)
Published	22 years, 7 months ago (Jan. 1, 2003)
Published Online	22 years, 7 months ago (Jan. 1, 2003)
Published Print	22 years, 7 months ago (Jan. 1, 2003)

Funders 0

None

BibTeX

@article{Tang_2003, title={A biochemical framework for RNA silencing in plants}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1048103}, DOI={10.1101/gad.1048103}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Tang, Guiliang and Reinhart, Brenda J. and Bartel, David P. and Zamore, Phillip D.}, year={2003}, month=jan, pages={49–63} }

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