DOI: 10.1101/gad.1040803. Translation elongation after assembly of ribosomes on the Cricket paralysis virus internal ribosomal entry site without initiation factors or initiator tRNA

Translation elongation after assembly of ribosomes on the Cricket paralysis virus internal ribosomal entry site without initiation factors or initiator tRNA

10.1101/gad.1040803

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Reconstitution of translation elongation from purified components confirmed that ribosomes that assembled on the Cricket paralysis virus intercistronic internal ribosomal entry site (IRES) without the involvement of initiation factors or initiator tRNA were active in elongation and are, therefore, true initiation complexes. The first elongation cycle occurred without peptide bond formation on 80S ribosomes that did not contain tRNA in the P site. It required elongation factors 1A and 2 and A site-cognate aminoacylated tRNA. Cycloheximide arrested ribosomes on the IRES only after two cycles of elongation, when the first deacylated tRNA reached the E-site after translocation from the A-site.

Bibliography

Pestova, T. V., & Hellen, C. U. T. (2003). Translation elongation after assembly of ribosomes on the Cricket paralysis virus internal ribosomal entry site without initiation factors or initiator tRNA. Genes & Development, 17(2), 181â186.

Authors 2

Tatyana V. Pestova (first)
Christopher U.T. Hellen (additional)

References 31 Referenced 212

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Dates

Type	When
Created	22 years, 7 months ago (Jan. 17, 2003, 10:42 p.m.)
Deposited	3 years, 9 months ago (Nov. 15, 2021, 10:11 p.m.)
Indexed	1 day, 3 hours ago (Aug. 20, 2025, 9:18 a.m.)
Issued	22 years, 7 months ago (Jan. 15, 2003)
Published	22 years, 7 months ago (Jan. 15, 2003)
Published Online	22 years, 7 months ago (Jan. 15, 2003)
Published Print	22 years, 7 months ago (Jan. 15, 2003)

Funders 0

None

BibTeX

@article{Pestova_2003, title={Translation elongation after assembly of ribosomes on the Cricket paralysis virus internal ribosomal entry site without initiation factors or initiator tRNA}, volume={17}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.1040803}, DOI={10.1101/gad.1040803}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Pestova, Tatyana V. and Hellen, Christopher U.T.}, year={2003}, month=jan, pages={181–186} }

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