CBP as a transcriptional coactivator of c-Myb.
10.1101/gad.10.5.528
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

CBP (CREB-binding protein) is a transcriptional coactivator of CREB (cAMP response element-binding) protein, which is directly phosphorylated by PKA (cAMP-dependent protein kinase A). CBP interacts with the activated phosphorylated form of CREB but not with the nonphosphorylated form. We report here that CBP is also a coactivator of the c-myb proto-oncogene product (c-Myb), which is a sequence-specific transcriptional activator. CBP directly binds to the region containing the transcriptional activation domain of c-Myb in a phosphorylation-independent manner in vitro. The domain of CBP that touches c-Myb is also required for binding to CREB. A c-Myb/CBP complex in vivo was demonstrated by a yeast two-hybrid assay. CBP stimulates the c-Myb-dependent transcriptional activation. Conversely, the expression of antisense RNA of CBP represses c-Myb-induced transcriptional activation. In addition, adenovirus EIA, which binds to CBP, inhibits c-Myb-induced transcriptional activation. Our data thus identify CBP as a coactivator of c-Myb. These results suggest that CBP functions as a coactivator for more transcriptional activators than were thought previously.

Bibliography

Dai, P., Akimaru, H., Tanaka, Y., Hou, D. X., Yasukawa, T., Kanei-Ishii, C., Takahashi, T., & Ishii, S. (1996). CBP as a transcriptional coactivator of c-Myb. Genes & Development, 10(5), 528–540.

Authors 8
  1. P Dai (first)
  2. H Akimaru (additional)
  3. Y Tanaka (additional)
  4. D X Hou (additional)
  5. T Yasukawa (additional)
  6. C Kanei-Ishii (additional)
  7. T Takahashi (additional)
  8. S Ishii (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:47 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued 29 years, 5 months ago (March 1, 1996)
Published 29 years, 5 months ago (March 1, 1996)
Published Online 29 years, 5 months ago (March 1, 1996)
Published Print 29 years, 5 months ago (March 1, 1996)
Funders 0

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@article{Dai_1996, title={CBP as a transcriptional coactivator of c-Myb.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.5.528}, DOI={10.1101/gad.10.5.528}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Dai, P and Akimaru, H and Tanaka, Y and Hou, D X and Yasukawa, T and Kanei-Ishii, C and Takahashi, T and Ishii, S}, year={1996}, month=mar, pages={528–540} }