Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation.
10.1101/gad.10.18.2368
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAFIIs). In vitro experiments have suggested that TAFIIs are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAFII, yeast TAFII90 (yTAFII90). We show that functional inactivation of yTAFII90 by temperature-sensitive mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAFII90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAFII90 is not required for transcription activation in general, and reveal linkages between TAF function and cell-cycle progression.

Bibliography

Apone, L. M., Virbasius, C. M., Reese, J. C., & Green, M. R. (1996). Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation. Genes & Development, 10(18), 2368–2380.

Authors 4
  1. L M Apone (first)
  2. C M Virbasius (additional)
  3. J C Reese (additional)
  4. M R Green (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:52 p.m.)
Indexed 2 weeks, 6 days ago (Aug. 5, 2025, 8:25 a.m.)
Issued 28 years, 11 months ago (Sept. 15, 1996)
Published 28 years, 11 months ago (Sept. 15, 1996)
Published Online 28 years, 11 months ago (Sept. 15, 1996)
Published Print 28 years, 11 months ago (Sept. 15, 1996)
Funders 0

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@article{Apone_1996, title={Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.18.2368}, DOI={10.1101/gad.10.18.2368}, number={18}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Apone, L M and Virbasius, C M and Reese, J C and Green, M R}, year={1996}, month=sep, pages={2368–2380} }