DOI: 10.1101/gad.10.18.2326. In vivo targeted mutagenesis of a regulatory element required for positioning the Hoxd-11 and Hoxd-10 expression boundaries.

In vivo targeted mutagenesis of a regulatory element required for positioning the Hoxd-11 and Hoxd-10 expression boundaries.

10.1101/gad.10.18.2326

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Vertebrate Hox genes are required for the proper organization of structures along the rostrocaudal axis. Hoxd-11 is expressed in the posterior part of the embryo, up to the level of prevertebra 27, and its expression boundary is reproduced by a Hoxd-11/lacZ transgene. Expression of this transgene anterior to prevertebra 27 is prevented by the silencing activity of a cis-acting element, region IX. Using transgenic mice, we show that Hoxd-11 repression by region IX is necessary to position the sacrum properly. This silencing activity depends on phylogenetically conserved sequences able to bind in vitro retinoic acid receptors and COUP-TFs. ES cells were used to generate mice carrying a subtle mutation that abolishes binding of nuclear receptors to region IX. Mutant mice display an anterior shift of their lumbosacral transition inherited as a codominant trait. In mutant embryos, expression of both Hoxd-11 and Hoxd-10 mRNAs in the prevertebral column is anteriorized. These results illustrate the sharing, in cis, of a single regulatory element in order to establish the expression boundaries of two neighboring Hoxd genes.

Bibliography

GÃ©rard, M., Chen, J. Y., Gronemeyer, H., Chambon, P., Duboule, D., & ZÃ¡kÃ¡ny, J. (1996). In vivo targeted mutagenesis of a regulatory element required for positioning the Hoxd-11 and Hoxd-10 expression boundaries. Genes & Development, 10(18), 2326â2334.

Authors 6

M Gérard (first)
J Y Chen (additional)
H Gronemeyer (additional)
P Chambon (additional)
D Duboule (additional)
J Zákány (additional)

References 33 Referenced 86

10.1126/science.2660260
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{'key': '2021111418395333000_10.18.2326.3', 'first-page': '4153', 'article-title': 'Chicken ovalbumin upstream promoter transcription factor (COUP-TF) dimers bind to different GGTCA response elements, allowing COUP-TF to repress hormonal induction of the vitamin D3, thyroid hormone, and retinoic acid receptors.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Chicken ovalbumin upstream promoter transcription factor (COUP-TF) dimers bind to different GGTCA response elements, allowing COUP-TF to repress hormonal induction of the vitamin D3, thyroid hormone, and retinoic acid receptors. (1992)
10.1242/dev.120.8.2187 / Development / Axial homeosis and appendicular skeleton defects in mice with a targeted disruption of Hoxd-11. (1994)
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{'key': '2021111418395333000_10.18.2326.6', 'first-page': '27', 'article-title': 'The in vitro development of blastocyst-derived embryonic stem cell lines: Formation of visceral yolk sac, blood islands and myocardium.', 'volume': '87', 'year': '1985', 'journal-title': 'J. Embryol. Exp. Morpholol.'} / J. Embryol. Exp. Morpholol. / The in vitro development of blastocyst-derived embryonic stem cell lines: Formation of visceral yolk sac, blood islands and myocardium. (1985)
Dollé, P., Dierich, A. Le Meur M. Schimmang, T. Schuhbaur, B. Chambon, P. and Duboule. D. 1993. Disruption of the Hoxd-13 gene induces localized heterochrony leading to mice with neotenic limbs. Cell 75: 43-1-441. (10.1016/0092-8674(93)90378-4)
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10.1242/dev.122.2.449 / Development / Functional cooperation between the non-paralogous genes Hoxa-10 and Hoxd-11 in the developing forelimb and axial skeleton. (1996)
10.1002/j.1460-2075.1993.tb06028.x / EMBO J. / Structure and activity of regulatory elements involved in the activation of the Hoxd-11 gene during late gastrulation. (1993)
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10.1006/dbio.1996.0137

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:52 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 11:18 a.m.)
Issued	28 years, 11 months ago (Sept. 15, 1996)
Published	28 years, 11 months ago (Sept. 15, 1996)
Published Online	28 years, 11 months ago (Sept. 15, 1996)
Published Print	28 years, 11 months ago (Sept. 15, 1996)

Funders 0

None

BibTeX

@article{G_rard_1996, title={In vivo targeted mutagenesis of a regulatory element required for positioning the Hoxd-11 and Hoxd-10 expression boundaries.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.18.2326}, DOI={10.1101/gad.10.18.2326}, number={18}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Gérard, M and Chen, J Y and Gronemeyer, H and Chambon, P and Duboule, D and Zákány, J}, year={1996}, month=sep, pages={2326–2334} }

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