DOI: 10.1101/gad.10.17.2189. The Caenorhabditis elegans gene lin-17, which is required for certain asymmetric cell divisions, encodes a putative seven-transmembrane protein similar to the Drosophila frizzled protein.

The Caenorhabditis elegans gene lin-17, which is required for certain asymmetric cell divisions, encodes a putative seven-transmembrane protein similar to the Drosophila frizzled protein.

10.1101/gad.10.17.2189

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Mutations in the gene lin-17 result in the disruption of a variety of asymmetric cell divisions in Caenorhabditis elegans. We have found that lin-17 encodes a protein with seven putative transmembrane domains. The LIN-17 protein is most similar to the Drosophila Frizzled protein and its vertebrate homologs. Studies using a lin-17-green fluorescent protein translational fusion indicate that lin-17 is expressed in mother cells before asymmetric cell divisions and in both daughter cells after the divisions. Our results suggest that lin-17 encodes a receptor that regulates the polarities of cells undergoing asymmetric cell divisions and raise the possibility that the LIN-17 protein acts as a receptor for the Wnt protein LIN-44, which also controls asymmetric cell divisions.

Bibliography

Sawa, H., Lobel, L., & Horvitz, H. R. (1996). The Caenorhabditis elegans gene lin-17, which is required for certain asymmetric cell divisions, encodes a putative seven-transmembrane protein similar to the Drosophila frizzled protein. Genes & Development, 10(17), 2189â2197.

Authors 3

H Sawa (first)
L Lobel (additional)
H R Horvitz (additional)

References 48 Referenced 171

10.1002/bies.950141103
10.1093/genetics/126.2.401 / Genetics / Molecular structure of frizzled, a Drosophila tissue polarity gene. (1990)
10.1051/medsci/20031967725
10.1126/science.8303295
10.1006/dbio.1995.1246
10.1016/S0021-9258(19)74025-3 / J. Biol. Chem. / Two homologs of the Drosophila polarity gene frizzled (fz) are widely expressed in mammalian tissues. (1992)
10.1016/0092-8674(95)90035-7
10.1038/377052a0
10.1038/335184a0
10.1146/annurev.bi.60.070191.003253
10.1093/genetics/110.1.17 / Genetics / Identification and characterization of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans. (1985)
10.1038/326259a0
10.1016/0378-1119(90)90224-F
Gubb, D. 1993. Genes controlling cellular polarity in Drosophila. Development (Suppl.) 269–277. (10.1242/dev.119.Supplement.269)
10.1038/373663a0
10.1242/dev.120.5.1035 / Development / The Caenorhabditis elegans gene lin-44 controls the polarity of asymmetric cell divisions. (1994)
10.1016/0092-8674(95)90238-4
10.1038/377627a0
10.1093/genetics/91.1.67 / Genetics / Nondisjunction mutants of the nematode C. elegans. (1979)
10.1016/0092-8674(92)90468-R
10.1093/genetics/142.1.205 / Genetics / Molecular analysis of EMS-induced frizzled mutations in Drosophila melanogaster. (1996)
10.1038/377624a0
10.1242/dev.121.12.4095 / Development / dishevelled is a component of the frizzled signaling pathway in Drosophila. (1995)
10.1016/0092-8674(87)90613-1
10.1016/0022-2836(82)90515-0
10.1016/0092-8674(95)90100-0
10.1002/j.1460-2075.1991.tb04966.x / EMBO J. / Efficient gene transfer in C. elegans: Extrachromosomal maintenance and integration of transforming sequences. (1991)
10.1038/367080a0
10.1016/0092-8674(92)90630-U
10.1242/dev.120.8.2175 / Development / The Caenorhabditis elegans NK-2 class homeoprotein CEH-22 is involved in combinatorial activation of gene expression in pharyngeal muscle. (1994)
10.1016/0925-4773(94)90026-4
10.1074/jbc.270.9.4705
10.1016/0092-8674(94)90112-0
10.1242/dev.115.2.475 / Development / Isolation and expression of two novel Wnt/wingless gene homologues in Drosophila. (1992)
Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
{'key': '2021111418393530000_10.17.2189.36', 'first-page': '1857', 'article-title': 'Two wnt genes in Caenorhabditis elegans.', 'volume': '8', 'year': '1993', 'journal-title': 'Oncogene'} / Oncogene / Two wnt genes in Caenorhabditis elegans. (1993)
10.1038/367076a0
10.1242/dev.121.10.3187 / Development / The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila. (1995)
10.1016/0012-1606(88)90414-9
10.1146/annurev.bi.63.070194.000533
10.1091/mbc.3.2.221
10.1242/dev.120.2.347 / Development / dishevelled is required during wingless signaling to establish both cell polarity and cell identity. (1994)
10.1038/329549a0
10.1038/338263a0
10.1074/jbc.271.8.4468
10.1002/aja.1001940405 / Dev. Dynamics / Regulation of anterior cell-specific mec-3 expression during asymmetric cell-division in C. elegans. (1992)
10.1016/0092-8674(94)90367-0
10.1006/geno.1995.1060

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:53 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:29 p.m.)
Issued	28 years, 11 months ago (Sept. 1, 1996)
Published	28 years, 11 months ago (Sept. 1, 1996)
Published Online	28 years, 11 months ago (Sept. 1, 1996)
Published Print	28 years, 11 months ago (Sept. 1, 1996)

Funders 0

None

BibTeX

@article{Sawa_1996, title={The Caenorhabditis elegans gene lin-17, which is required for certain asymmetric cell divisions, encodes a putative seven-transmembrane protein similar to the Drosophila frizzled protein.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.17.2189}, DOI={10.1101/gad.10.17.2189}, number={17}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Sawa, H and Lobel, L and Horvitz, H R}, year={1996}, month=sep, pages={2189–2197} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        15
      ]
    ],
    "date-time": "2025-07-15T03:29:13Z",
    "timestamp": 1752550153972
  },
  "reference-count": 48,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "17",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        9,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Mutations in the gene lin-17 result in the disruption of a variety of asymmetric cell divisions in Caenorhabditis elegans. We have found that lin-17 encodes a protein with seven putative transmembrane domains. The LIN-17 protein is most similar to the Drosophila Frizzled protein and its vertebrate homologs. Studies using a lin-17-green fluorescent protein translational fusion indicate that lin-17 is expressed in mother cells before asymmetric cell divisions and in both daughter cells after the divisions. Our results suggest that lin-17 encodes a receptor that regulates the polarities of cells undergoing asymmetric cell divisions and raise the possibility that the LIN-17 protein acts as a receptor for the Wnt protein LIN-44, which also controls asymmetric cell divisions.</jats:p>",
  "DOI": "10.1101/gad.10.17.2189",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "2189-2197",
  "source": "Crossref",
  "is-referenced-by-count": 171,
  "title": "The Caenorhabditis elegans gene lin-17, which is required for certain asymmetric cell divisions, encodes a putative seven-transmembrane protein similar to the Drosophila frizzled protein.",
  "prefix": "10.1101",
  "volume": "10",
  "author": [
    {
      "given": "H",
      "family": "Sawa",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "L",
      "family": "Lobel",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "H R",
      "family": "Horvitz",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1996,
        9,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111418393530000_10.17.2189.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bies.950141103"
    },
    {
      "key": "2021111418393530000_10.17.2189.2",
      "doi-asserted-by": "crossref",
      "first-page": "401",
      "DOI": "10.1093/genetics/126.2.401",
      "article-title": "Molecular structure of frizzled, a Drosophila tissue polarity gene.",
      "volume": "126",
      "year": "1990",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111418393530000_10.17.2189.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1051/medsci/20031967725"
    },
    {
      "key": "2021111418393530000_10.17.2189.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8303295"
    },
    {
      "key": "2021111418393530000_10.17.2189.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1995.1246"
    },
    {
      "key": "2021111418393530000_10.17.2189.6",
      "doi-asserted-by": "crossref",
      "first-page": "25202",
      "DOI": "10.1016/S0021-9258(19)74025-3",
      "article-title": "Two homologs of the Drosophila polarity gene frizzled (fz) are widely expressed in mammalian tissues.",
      "volume": "267",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418393530000_10.17.2189.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90035-7"
    },
    {
      "key": "2021111418393530000_10.17.2189.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377052a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/335184a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.60.070191.003253"
    },
    {
      "key": "2021111418393530000_10.17.2189.11",
      "doi-asserted-by": "crossref",
      "first-page": "17",
      "DOI": "10.1093/genetics/110.1.17",
      "article-title": "Identification and characterization of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans.",
      "volume": "110",
      "year": "1985",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111418393530000_10.17.2189.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/326259a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(90)90224-F"
    },
    {
      "key": "2021111418393530000_10.17.2189.14",
      "doi-asserted-by": "crossref",
      "unstructured": "Gubb, D. 1993. Genes controlling cellular polarity in Drosophila. Development (Suppl.) 269\u2013277.",
      "DOI": "10.1242/dev.119.Supplement.269"
    },
    {
      "key": "2021111418393530000_10.17.2189.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/373663a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.16",
      "doi-asserted-by": "crossref",
      "first-page": "1035",
      "DOI": "10.1242/dev.120.5.1035",
      "article-title": "The Caenorhabditis elegans gene lin-44 controls the polarity of asymmetric cell divisions.",
      "volume": "120",
      "year": "1994",
      "journal-title": "Development"
    },
    {
      "key": "2021111418393530000_10.17.2189.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90238-4"
    },
    {
      "key": "2021111418393530000_10.17.2189.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377627a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.19",
      "doi-asserted-by": "crossref",
      "first-page": "67",
      "DOI": "10.1093/genetics/91.1.67",
      "article-title": "Nondisjunction mutants of the nematode C. elegans.",
      "volume": "91",
      "year": "1979",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111418393530000_10.17.2189.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90468-R"
    },
    {
      "key": "2021111418393530000_10.17.2189.21",
      "doi-asserted-by": "crossref",
      "first-page": "205",
      "DOI": "10.1093/genetics/142.1.205",
      "article-title": "Molecular analysis of EMS-induced frizzled mutations in Drosophila melanogaster.",
      "volume": "142",
      "year": "1996",
      "journal-title": "Genetics"
    },
    {
      "key": "2021111418393530000_10.17.2189.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377624a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.23",
      "doi-asserted-by": "crossref",
      "first-page": "4095",
      "DOI": "10.1242/dev.121.12.4095",
      "article-title": "dishevelled is a component of the frizzled signaling pathway in Drosophila.",
      "volume": "121",
      "year": "1995",
      "journal-title": "Development"
    },
    {
      "key": "2021111418393530000_10.17.2189.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90613-1"
    },
    {
      "key": "2021111418393530000_10.17.2189.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(82)90515-0"
    },
    {
      "key": "2021111418393530000_10.17.2189.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90100-0"
    },
    {
      "key": "2021111418393530000_10.17.2189.27",
      "doi-asserted-by": "crossref",
      "first-page": "3959",
      "DOI": "10.1002/j.1460-2075.1991.tb04966.x",
      "article-title": "Efficient gene transfer in C. elegans: Extrachromosomal maintenance and integration of transforming sequences.",
      "volume": "10",
      "year": "1991",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418393530000_10.17.2189.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/367080a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90630-U"
    },
    {
      "key": "2021111418393530000_10.17.2189.30",
      "doi-asserted-by": "crossref",
      "first-page": "2175",
      "DOI": "10.1242/dev.120.8.2175",
      "article-title": "The Caenorhabditis elegans NK-2 class homeoprotein CEH-22 is involved in combinatorial activation of gene expression in pharyngeal muscle.",
      "volume": "120",
      "year": "1994",
      "journal-title": "Development"
    },
    {
      "key": "2021111418393530000_10.17.2189.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0925-4773(94)90026-4"
    },
    {
      "key": "2021111418393530000_10.17.2189.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.9.4705"
    },
    {
      "key": "2021111418393530000_10.17.2189.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90112-0"
    },
    {
      "key": "2021111418393530000_10.17.2189.34",
      "doi-asserted-by": "crossref",
      "first-page": "475",
      "DOI": "10.1242/dev.115.2.475",
      "article-title": "Isolation and expression of two novel Wnt/wingless gene homologues in Drosophila.",
      "volume": "115",
      "year": "1992",
      "journal-title": "Development"
    },
    {
      "key": "2021111418393530000_10.17.2189.35",
      "unstructured": "Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."
    },
    {
      "key": "2021111418393530000_10.17.2189.36",
      "first-page": "1857",
      "article-title": "Two wnt genes in Caenorhabditis elegans.",
      "volume": "8",
      "year": "1993",
      "journal-title": "Oncogene"
    },
    {
      "key": "2021111418393530000_10.17.2189.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/367076a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.38",
      "doi-asserted-by": "crossref",
      "first-page": "3187",
      "DOI": "10.1242/dev.121.10.3187",
      "article-title": "The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila.",
      "volume": "121",
      "year": "1995",
      "journal-title": "Development"
    },
    {
      "key": "2021111418393530000_10.17.2189.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(88)90414-9"
    },
    {
      "key": "2021111418393530000_10.17.2189.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.63.070194.000533"
    },
    {
      "key": "2021111418393530000_10.17.2189.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.3.2.221"
    },
    {
      "key": "2021111418393530000_10.17.2189.42",
      "doi-asserted-by": "crossref",
      "first-page": "347",
      "DOI": "10.1242/dev.120.2.347",
      "article-title": "dishevelled is required during wingless signaling to establish both cell polarity and cell identity.",
      "volume": "120",
      "year": "1994",
      "journal-title": "Development"
    },
    {
      "key": "2021111418393530000_10.17.2189.43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/329549a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/338263a0"
    },
    {
      "key": "2021111418393530000_10.17.2189.45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.8.4468"
    },
    {
      "key": "2021111418393530000_10.17.2189.46",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1002/aja.1001940405",
      "article-title": "Regulation of anterior cell-specific mec-3 expression during asymmetric cell-division in C. elegans.",
      "volume": "194",
      "year": "1992",
      "journal-title": "Dev. Dynamics"
    },
    {
      "key": "2021111418393530000_10.17.2189.47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90367-0"
    },
    {
      "key": "2021111418393530000_10.17.2189.48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1995.1060"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.10.17.2189",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T02:53:13Z",
    "timestamp": 1636944793000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.10.17.2189"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        9,
        1
      ]
    ]
  },
  "references-count": 48,
  "journal-issue": {
    "issue": "17",
    "published-print": {
      "date-parts": [
        [
          1996,
          9,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.10.17.2189"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.10.17.2189",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1996,
        9,
        1
      ]
    ]
  }
}