DOI: 10.1101/gad.10.15.1880. A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway.

A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway.

10.1101/gad.10.15.1880

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

A functional assay to clone mouse mesoderm inducers has identified the mouse gene Mad related 2 (Madr2). Madr2 induces dorsal mesoderm from Xenopus ectoderm and can mimic the organizer in recruiting neighboring cells into a second axis. By analyzing the expression of a lacZ/Madr2 fusion protein, we find Madr2 confined to the nucleus in the deep, anterior cells of the second axis, whereas in epidermal and more posterior cells the protein is cytoplasmically localized. This context-dependent nuclear localization suggests that in certain regions of the embryo, Madr2 responds to a localized signal and amplifies this signal to form the second axis. Furthermore, although Madr2 remains unlocalized in ectodermal explants, addition of activin enhances the concentration of Madr2 in the nucleus. Significantly, a functional lacZ fusion to a carboxy-terminal portion of Madr2 is nuclear localized even in the absence of activin. This indicates that Madr2 contains a domain that can activate downstream components and a repressive domain that anchors the protein in the cytoplasm. Nuclear localization of Madr2 in response to activin, and the activin-like phenotypes induced by overexpression of Madr2, indicate that Madr2 is a signal transduction component that mediates the activity of activin.

Bibliography

Baker, J. C., & Harland, R. M. (1996). A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway. Genes & Development, 10(15), 1880â1889.

Authors 2

J C Baker (first)
R M Harland (additional)

References 62 Referenced 275

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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:52 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	29 years ago (Aug. 1, 1996)
Published	29 years ago (Aug. 1, 1996)
Published Online	29 years ago (Aug. 1, 1996)
Published Print	29 years ago (Aug. 1, 1996)

Funders 0

None

BibTeX

@article{Baker_1996, title={A novel mesoderm inducer, Madr2, functions in the activin signal transduction pathway.}, volume={10}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.10.15.1880}, DOI={10.1101/gad.10.15.1880}, number={15}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Baker, J C and Harland, R M}, year={1996}, month=aug, pages={1880–1889} }

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